Evidence map›Paper›PMID 32805500›Full record

ArticleBiomaterials2020

A sequential 3D bioprinting and orthogonal bioconjugation approach for precision tissue engineering.

Claire Yu, Kathleen L Miller, Jacob Schimelman, Pengrui Wang, Wei Zhu, Xuanyi Ma, Min Tang, Shangting You, Deepak Lakshmipathy, Frank He and 1 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomaterials, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
2.4field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed, 47 citations in OpenAlex.

  1. Article
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  8. Lithography-based 3D printing of hydrogels.Nature reviews bioengineering · 2025
    Article
  9. Review
  10. Article
  11. Article
  12. Article
  13. Review
  14. Article
  15. Light-based vat-polymerization bioprinting.Nature reviews. Methods primers · 2023
    Article
  16. Review
  17. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 1 institution in 1 country.

Claire YuDepartment of NanoEngineering, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA, 92093, USA.
Kathleen L MillerDepartment of NanoEngineering, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA, 92093, USA.
Jacob SchimelmanDepartment of NanoEngineering, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA, 92093, USA.
Pengrui WangMaterials Science and Engineering Program, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA, 92093, USA.
Wei ZhuDepartment of NanoEngineering, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA, 92093, USA.
Xuanyi MaDepartment of Bioengineering, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA, 92093, USA.
Min TangDepartment of NanoEngineering, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA, 92093, USA.
Shangting YouDepartment of NanoEngineering, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA, 92093, USA.
Deepak LakshmipathyDepartment of NanoEngineering, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA, 92093, USA.
Frank HeDepartment of Bioengineering, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA, 92093, USA.
Shaochen ChenDepartment of NanoEngineering, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA, 92093, USA; Materials Science and Engineering Program, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA, 92093, USA; Department of Bioengineering, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA, 92093, USA; Chemical Engineering Program, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA, 92093, USA. Electronic address: chen168@eng.ucsd.edu.
University of California, San Diego · US

Funding

U of Calif, San Diego Neuroscience Microscopy ImagingP30NS047101 · NINDS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI GLEESON, JOSEPH G, ZHENG, BINHAI · 2003 to 2022
$9.0M
Rapid 3D bioprinting of biomimetic vascularized tissue constructsR01EB021857 · NIBIB · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI CHEN, SHAOCHEN, DOKMECI, MEHMET REMZI · 2016 to 2019
$2.1M
Rapid 3D-printing of Multi-functional Adaptive Nerve ConduitsR33HD090662 · NICHD · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI CHEN, SHAOCHEN · 2019 to 2021
$1.1M
Stem Cell-based Human Placenta-on-a-Chip Using 3D BioprintingR21HD100132 · NICHD · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI CHEN, SHAOCHEN, LAURENT, LOUISE CHANG · 2019 to 2020
$656k
3D Printing of Precision Scaffolds for Volumetric Muscle Tissue RegenerationR21AR074763 · NIAMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI CHEN, SHAOCHEN · 2019 to 2020
$355k
A stem cell based optic nerve model for studies of axon guidance and regenerationR21EY031122 · NEI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI CHEN, SHAOCHEN, WAHLIN, KARL J · 2019 to 2019
$299k
NEI NIH HHS R21 EY031122NIAMS NIH HHS R21 AR074763NIBIB NIH HHS R01 EB021857NICHD NIH HHS R21 HD100132NICHD NIH HHS R33 HD090662NINDS NIH HHS P30 NS047101
6 · The paper itself

Abstract

Recent advances in 3D bioprinting have transformed the tissue engineering landscape by enabling the controlled placement of cells, biomaterials, and bioactive agents for the biofabrication of living tissues and organs. However, the application of 3D bioprinting is limited by the availability of cytocompatible and printable biomaterials that recapitulate properties of native tissues. Here, we developed an integrated 3D projection bioprinting and orthogonal photoconjugation platform for precision tissue engineering of tailored microenvironments. By using a photoreactive thiol-ene gelatin bioink, soft hydrogels can be bioprinted into complex geometries and photopatterned with bioactive moieties in a rapid and scalable manner via digital light projection (DLP) technology. This enables localized modulation of biophysical properties such as stiffness and microarchitecture as well as precise control over spatial distribution and concentration of immobilized functional groups. As such, well-defined properties can be directly incorporated using a single platform to produce desired tissue-specific functions within bioprinted constructs. We demonstrated high viability of encapsulated endothelial cells and human cardiomyocytes using our dual process and fabricated tissue constructs functionalized with VEGF peptide mimics to induce guided endothelial cell growth for programmable vascularization. This work represents a pivotal step in engineering multifunctional constructs with unprecedented control, precision, and versatility for the rational design of biomimetic tissues.

Indexed as

BioprintingEndothelial CellsGelatinHumansHydrogelsPrinting, Three-DimensionalTissue EngineeringTissue ScaffoldsGelatinHydrogels3D bioprintingBiomaterialsOrthogonal photopatterningThiol-ene click chemistryTissue engineering

Identifiers

PMID32805500
PMCPMC7489302
OpenAlexW3047715837

What OpenQuestion holds

Textmetadata
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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.