ArticlePloS one2020
CRISPR-induced indels and base editing using the Staphylococcus aureus Cas9 in potato.
Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed, 48 citations in OpenAlex.
- Advances in CRISPR multiplex gene editing to map and modify stress-responsive transcription factor networks for crop improvement.Plant cell reports · 2026Review
- Boosting cytosine base editing in potato through synergistic optimization.Horticulture research · 2026Article
- CRISPR-Cas applications in agriculture and plant research.Nature reviews. Molecular cell biology · 2025Review
- Potato: from functional genomics to genetic improvement.Molecular horticulture · 2024Review
- Targeted mutagenesis with sequence-specific nucleases for accelerated improvement of polyploid crops: Progress, challenges, and prospects.The plant genome · 2023Review
- Tools and targets: The dual role of plant viruses in CRISPR-Cas genome editing.The plant genome · 2023Review
- Enhancing the quality of staple food crops through CRISPR/Cas-mediated site-directed mutagenesis.Planta · 2023Review
- Multiplex CRISPR-Cas9 Gene-Editing Can Deliver Potato Cultivars with Reduced Browning and Acrylamide.Plants (Basel, Switzerland) · 2023Article
- Cytosine base editors optimized for genome editing in potato protoplasts.Frontiers in genome editing · 2023Article
- CRISPR/Cas genome editing system and its application in potato.Frontiers in genetics · 2023Review
- Review
- Genome editing (CRISPR-Cas)-mediated virus resistance in potato (Solanum tuberosum L.).Molecular biology reports · 2022Review
- CRISPR/Cas-mediated knockdown of vacuolar invertase gene expression lowers the cold-induced sweetening in potatoes.Planta · 2022Article
- Mechanistic Concept of Physiological, Biochemical, and Molecular Responses of the Potato Crop to Heat and Drought Stress.Plants (Basel, Switzerland) · 2022Review
- Genome Editing of Veterinary Relevant Mycoplasmas Using a CRISPR-Cas Base Editor System.Applied and environmental microbiology · 2022Article
- Advances in Delivery Mechanisms of CRISPR Gene-Editing Reagents in Plants.Frontiers in genome editing · 2022Review
- CRISPR/Cas Genome Editing in Potato: Current Status and Future Perspectives.Frontiers in genetics · 2022Article
- Comprehending the evolution of gene editing platforms for crop trait improvement.Frontiers in genetics · 2022Review
- CRISPR/Cas9-mediated fine-tuning of miRNA expression in tetraploid potato.Horticulture research · 2022Article
- Precise plant genome editing using base editors and prime editors.Nature plants · 2021Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genome editing is now widely used in plant science for both basic research and molecular crop breeding. The clustered regularly interspaced short palindromic repeats (CRISPR) technology, through its precision, high efficiency and versatility, allows for editing of many sites in plant genomes. This system has been highly successful to produce knock-out mutants through the introduction of frameshift mutations due to error-prone repair pathways. Nevertheless, recent new CRISPR-based technologies such as base editing and prime editing can generate precise and on demand nucleotide conversion, allowing for fine-tuning of protein function and generating gain-of-function mutants. However, genome editing through CRISPR systems still have some drawbacks and limitations, such as the PAM restriction and the need for more diversity in CRISPR tools to mediate different simultaneous catalytic activities. In this study, we successfully used the CRISPR-Cas9 system from Staphylococcus aureus (SaCas9) for the introduction of frameshift mutations in the tetraploid genome of the cultivated potato (Solanum tuberosum). We also developed a S. aureus-cytosine base editor that mediate nucleotide conversions, allowing for precise modification of specific residues or regulatory elements in potato. Our proof-of-concept in potato expand the plant dicot CRISPR toolbox for biotechnology and precision breeding applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.