Evidence map›Paper›PMID 32803953›Full record

ArticleACS combinatorial science2020

Multiplexed Enzyme Activity-Based Probe Display via Hybridization.

Valerie Cavett, Brian M Paegel

Open access · greenAbstract read
In one paragraph

Article in ACS combinatorial science, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.1field-weighted citation impact, top 60% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 2 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Valerie CavettDepartment of Pharmaceutical Sciences, University of California, Irvine, California 92617, United States.ORCID 0000-0003-2859-6355
Brian M PaegelDepartment of Pharmaceutical Sciences, University of California, Irvine, California 92617, United States.ORCID 0000-0002-6531-6693
University of California, Irvine · US

Funding

High-Throughput Droplet-Scale Functional Screening of DNA-Encoded Combinatorial LibrariesR01GM120491 · NIGMS · UNIVERSITY OF CALIFORNIA-IRVINE · PI PAEGEL, BRIAN M · 2017 to 2020
$1.4M
NIGMS NIH HHS R01 GM120491
6 · The paper itself

Abstract

Emulsions offer the means to miniaturize and parallelize high-throughput screening but require a robust method to localize activity-based fluorescent probes in each droplet. Multiplexing probes in droplets is impractical, though highly desirable for identifying library members that possess very specific activity. Here, we present multiplexed probe immobilization on library beads for emulsion screening. During library bead preparation, we quantitated ∼10

Indexed as

ChymotrypsinCombinatorial Chemistry TechniquesDNADNA PrimersEmulsionsEnzyme ActivationFlow CytometryFluorescent DyesGene LibraryHigh-Throughput Screening AssaysIn Situ Hybridization, FluorescenceMultiplex Polymerase Chain ReactionMutationSurface PropertiesTrypsinChymotrypsinDNADNA PrimersEmulsionsFluorescent DyesTrypsinbeadsdirected evolutionemulsionproteasescreening

Identifiers

PMID32803953
PMCPMC7655629
OpenAlexW3060588429

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.