Evidence map›Paper›PMID 32803867›Full record

ArticleJournal of cellular and molecular medicine2020

BDNF promoted osteoblast migration and fracture healing by up-regulating integrin β1 via TrkB-mediated ERK1/2 and AKT signalling.

Zitao Zhang, Polu Hu, Zhen Wang, Xusheng Qiu, Yixin Chen

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of cellular and molecular medicine, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.

0numbers the graph read from it
0cells of the map it votes in
27citing papers in PubMed
1.4field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

27 citing papers in PubMed, 31 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Zitao ZhangDepartment of Orthopedics, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, China.ORCID 0000-0002-5862-7530
Polu HuNanjing Red Cross Blood Center, Nanjing, China.
Zhen WangDepartment of Orthopedics, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, China.
Xusheng QiuDepartment of Orthopedics, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, China.
Yixin ChenDepartment of Orthopedics, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, China.
Nanjing Drum Tower Hospital · CNBeijing Red Cross Blood Center · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Brain-derived neurotrophic factor (BDNF) has been reported to participate in fracture healing, whereas the mechanism is still unclear. Since osteoblast migration is important for fracture healing, investigating effects of BDNF on osteoblasts migration may help to reveal its mechanism. Here, MC3T3-E1 cells were used in vitro while closed femur fracture mice were applied in vivo. Cells migration was assessed with Transwell assay. The protein expression was analysed by immunoblotting. X-ray and Micro-CT were performed at different time after fracture. Our results showed that BDNF promoted MC3T3-E1 cells migration, integrin β1 expression and ERK1/2 and AKT phosphorylation. K252a, a specific inhibitor for TrkB, suppressed BDNF-induced migration, integrin β1 expression and activation of ERK1/2 and AKT. PD98059 (an ERK1/2 inhibitor) and LY294002 (an AKT inhibitor) both inhibited BDNF-induced migration and integrin β1 expression while integrin β1 blocking antibody only suppressed cell migration. X-ray and Micro-CT analyses showed that the adenoviral carried integrin β1 shRNA group had slower fracture healing at 7 and 21 days, but not 35 days compared to the control group. Thus, we proposed that BDNF stimulated MC3T3-E1 cells migration by up-regulating integrin β1 via TrkB mediated ERK1/2 and AKT signalling, and this may help to enhance the fracture healing.

Indexed as

AnimalsBrain-Derived Neurotrophic FactorCell Line, TransformedCell MovementCore Binding Factor Alpha 1 SubunitFemoral FracturesFracture HealingIntegrin beta1MaleMAP Kinase Signaling SystemMembrane GlycoproteinsMiceMice, Inbred C57BLOsteoblastsProtein Kinase InhibitorsProtein-Tyrosine KinasesAkt1 protein, mouseBdnf protein, mouseBrain-Derived Neurotrophic FactorCore Binding Factor Alpha 1 SubunitIntegrin beta1Itgb1 protein, mouseMembrane GlycoproteinsNtrk2 protein, mouseProtein Kinase InhibitorsProtein-Tyrosine KinasesProto-Oncogene Proteins c-aktReceptor, trkBRNA, Small InterferingRunx2 protein, mousebrain-derived neurotrophic factorfracture healingintegrin β1MC3T3-E1 cells migration

Identifiers

PMID32803867
PMCPMC7521296
OpenAlexW3049634485

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.