ArticleGenes & genomics2020
Molecular characterization of Arabidopsis thaliana LSH1 and LSH2 genes.
Article in Genes & genomics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 21 citations in OpenAlex.
- Genomic and Functional Analysis of the ALOG Gene Family inPlants (Basel, Switzerland) · 2026Article
- Characterization and Expression Analysis of thePlants (Basel, Switzerland) · 2025Article
- Genome-wide identification and characterization ofFrontiers in plant science · 2025Article
- Review
- The ALOG domain defines a family of plant-specific transcription factors acting during Arabidopsis flower development.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Screening and identification of photoresponse factors in kiwifruit (Actinidia arguta) development.Molecular biology reports · 2024Article
- Expression profiling of ALOG family genes during inflorescence development and abiotic stress responses in rice (Frontiers in genetics · 2024Article
- Article
- Identification and candidate gene mining of HvSS1, a novel qualitative locus on chromosome 6H, regulating the uppermost internode elongation in barley (Hordeum vulgare L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2021Article
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundArabidopsis thaliana genome encodes ten DUF640 (short for domain of unknown function 640)/ALOG (short for Arabidopsis LSH1 and Oryza G1) proteins, also known as light-dependent short hypocotyl (LSH) proteins. While some of the LSH genes regulate organ boundary determination and shade avoidance response, the function of most of these genes remains largely unknown.
objectiveIn this study, we aimed to characterize the function of AtLSH1 and AtLSH2 in Arabidopsis.
methodsWe overexpressed AtLSH1 and AtLSH2 (with or without the FLAG tag) in Arabidopsis Col-0 plants under the control of the 35S promoter. We also generated knockout or knockdown lines of these genes by miRNA-induced gene silencing (MIGS). We conducted intensive phenotypic analysis of these transgenic lines, and finally performed RNA-seq analysis of two AtLSH2 overexpression (OX) lines.
resultsAlthough AtLSH1 and AtLSH2 amino acid sequences showed high similarly, AtLSH2-OX lines showed much higher levels of their transcripts than those of AtLSH1-OX lines. Additionally, overexpression of AtLSH1 and AtLSH2 greatly inhibited hypocotyl elongation in a light-independent manner, and reduced both vegetative and reproductive growth. However, knockout or knockdown of both these AtLSH genes did not affect plant phenotype. Gene Ontology (GO) analysis of differentially expressed genes (DEGs) identified by RNA-seq revealed enrichment of the GO term 'response to stimulus', included phytohormone-responsive genes; however, genes responsible for the abnormal phenotypes of AtLSH2-OX lines could not be identified.
conclusionAlthough our data revealed no close association between light and phytohormone signaling components, overexpression of AtLSH1 and AtLSH2 greatly reduced vegetative and reproductive growth of Arabidopsis plants. This property could be used to generate new plants by regulating expression of AtLSH1 and AtLSH2.
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