ArticleTissue engineering and regenerative medicine2020
Combined Delivery of Two Different Bioactive Factors Incorporated in Hydroxyapatite Microcarrier for Bone Regeneration.
Article in Tissue engineering and regenerative medicine, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 23 citations in OpenAlex.
- Advances and Clinical Translation Potentials of Functional Nanomaterials in Tissue Engineering.Bioengineering (Basel, Switzerland) · 2026Review
- Harnessing and Optimizing α-TCP for Oral Tissue Engineering and Regenerative Dentistry.International dental journal · 2026Review
- Histological and Immunohistochemical Evaluation of Rh-BMP2: Effect on Gingival Healing Acceleration and Proliferation of Human Epithelial Cells.Life (Basel, Switzerland) · 2024Article
- Comparison of the osteogenic effects of demineralized dentin matrix and acellular dentin matrix.Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology · 2024Article
- Recent advancement in vascularized tissue-engineered bone based on materials design and modification.Materials today. Bio · 2023Review
- Calcium Phosphate Delivery Systems for Regeneration and Biomineralization of Mineralized Tissues of the Craniofacial Complex.Molecular pharmaceutics · 2023Review
- Bone Regeneration with 3D-Printed Hybrid Bone Scaffolds in a Canine Radial Bone Defect Model.Tissue engineering and regenerative medicine · 2022Article
- In vitro photodynamic therapy of methylene blue-loaded acetyl resistant starch nanoparticles.Biomaterials research · 2022Article
- Electrically controlled mRNA delivery using a polypyrrole-graphene oxide hybrid film to promote osteogenic differentiation of human mesenchymal stem cells.Nano research · 2022Article
- Mucosal Vaccine Delivery Using Mucoadhesive Polymer Particulate Systems.Tissue engineering and regenerative medicine · 2021Review
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Authors and funding
7 authors at 2 institutions in 3 countries.
Funding
Abstract
backgroundThe delivery of growth factors using a carrier system presents a promising and innovative tool in tissue engineering and dentistry today. Two of the foremost bioactive factors, bone morphogenetic protein-2 and vascular endothelial growth factor (VEGF), are widely applied using a ceramic scaffold. The aim of this study was to determine the use of hydroxyapatite microcarrier (MC) for dual delivery of osteogenic and angiogenic factors to accelerate hard tissue regeneration during the regenerative process.
methodsTwo MCs of different sizes were fabricated by emulsification of gelatin and alpha-tricalcium phosphate (α-TCP). The experimental group was divided based on the combination of MC size and growth factors. For investigating the in vitro properties, rat mesenchymal stem cells (rMSCs) were harvested from bone marrow of the femur and tibia. For in vivo experiments, MC with/without growth factors was applied into the standardized, 5-mm diameter defects, which were made bilaterally on the parietal bone of the rat. The animals were allowed to heal for 8 weeks, and samples were harvested and analyzed by micro-computed tomography and histology.
resultsImproved proliferation of rat mesenchymal stem cells was observed with VEGF loaded MC. For osteogenic differentiation, dual growth factors delivered by MC showed higher osteogenic gene expression, alkaline phosphatse production and calcium deposition. The in vivo results revealed statistically significant increase in new bone formation when dual growth factors were delivered by MC. Dual growth factors administered on a calcium phosphate matrix showed significantly enhanced osteogenic potential.
conclusionWe propose this system has potential clinical utility in providing solutions for craniofacial bone defects, with the added benefit of early availability.
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