ArticleWorld journal of microbiology & biotechnology2020
The development of an efficient RNAi system based on Agrobacterium-mediated transformation approach for studying functional genomics in medical fungus Wolfiporia cocos.
Article in World journal of microbiology & biotechnology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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3 citing papers in PubMed, 11 citations in OpenAlex.
- Establishment of an efficient Agrobacterium tumefaciens-mediated transformation system for an Armillaria species, a host of the fully mycoheterotrophic plant Gastrodia elata.Folia microbiologica · 2025Article
- Research hotspots and emerging trends in growth and development of macrofungi: a bibliometric review based on CiteSpace analysis.World journal of microbiology & biotechnology · 2024Review
- Device-Controlled Microcondensation for Spatially Confined On-Tissue Digests in MALDI Imaging ofPharmaceuticals (Basel, Switzerland) · 2022Article
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5 authors at 1 institution in 1 country.
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Abstract
Genetic transformation methods reported for Wolfiporia cocos are limited. In this study, we describe an efficient RNA interference (RNAi) system based on Agrobacterium-mediated transformation approach in W. cocos for the first time. Actively growing mycelial plugs were used as recipients for transformation using endogenous orotidine-5'-phosphate decarboxylase gene (URA3) as both a selective marker and a silencing gene, under the control of the dual promoters of Legpd and Leactin from Lentinula edodes and the single promoter of Wcgpd from W. cocos, respectively. The results showed that both the two kinds of promoters effectively drive the expression of URA3 gene, and the URA3-silenced transformants could be selected on CYM medium containing 5'-fluoroorotic acid. In addition, silencing URA3 gene has no effect on the growth of W. cocos hyphae. The incomplete silencing of the URA3 locus was also observed in this study. This study will promote further study on the mechanism of substrate degradation, sclerotial formation, and biosynthesis network of pharmacological compounds in W. cocos.
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