ArticlePlant molecular biology2020
Host-induced silencing of the Colletotrichum gloeosporioides conidial morphology 1 gene (CgCOM1) confers resistance against Anthracnose disease in chilli and tomato.
Article in Plant molecular biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 43 citations in OpenAlex.
- Molecular mechanisms and agronomic strategies for thermotolerance in chili pepper (Capsicum annuum L.).Plant cell reports · 2026Review
- RNA interference mediated disease resistance in vegetable crops: fundamental discovery to current trends.Molecular biology reports · 2026Review
- Nano-Enabled Precision Management of Plant Anthracnose: Mechanisms of Action, Application Advances, and Future Perspectives.Journal of fungi (Basel, Switzerland) · 2026Review
- Sprayable dsRNA for integrated crop defense: mechanisms, delivery strategies, and translational challenges.Molecular biology reports · 2026Review
- Cross-kingdom RNAi: a universal mechanism of inter-organismal communication with many unknowns.Journal of experimental botany · 2026Review
- Plant Pathogenic and EndophyticMicroorganisms · 2025Review
- Current status and future trends of eco-friendly management of postharvest fungal decays in tomato fruit.NPJ science of food · 2025Review
- Strategies to develop climate-resilient chili peppers: transcription factor optimization through genome editing.Planta · 2025Review
- Molecular Insights into the Role of the MET30 Protein and Its WD40 Domain inJournal of fungi (Basel, Switzerland) · 2025Article
- Article
- Green Management of Postharvest Anthracnose Caused byJournal of fungi (Basel, Switzerland) · 2023Review
- Appressoria-Small but Incredibly Powerful Structures in Plant-Pathogen Interactions.International journal of molecular sciences · 2023Review
- Regulation and safety measures for nanotechnology-based agri-products.Frontiers in genome editing · 2023Review
- Management of Post-Harvest Anthracnose: Current Approaches and Future Perspectives.Plants (Basel, Switzerland) · 2022Review
- Concepts and considerations for enhancing RNAi efficiency in phytopathogenic fungi for RNAi-based crop protection using nanocarrier-mediated dsRNA delivery systems.Frontiers in fungal biology · 2022Review
- Small RNA-based plant protection against diseases.Frontiers in plant science · 2022Review
- Functional validation of pathogenicity genes in rice sheath blight pathogen Rhizoctonia solani by a novel host-induced gene silencing system.Molecular plant pathology · 2021Article
- RNAi-mediated silencing of3 Biotech · 2021Article
- Strategies to combat the problem of yam anthracnose disease: Status and prospects.Molecular plant pathology · 2021Review
- Development of an efficient Tef-1α RNA hairpin structure to efficient management of Lasiodiplodia theobromae and Neofusicoccum parvum.Scientific reports · 2021Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
key messageHost mediated silencing of COM1 gene of Colletotrichum gloeosporioides disables appressorial differentiation and effectively prevents the development of Anthracnose disease in chilli and tomato. Anthracnose disease is caused by the ascomycetes fungal species Colletotrichum, which is responsible for heavy yield losses in chilli and tomato worldwide. Conventionally, harmful pesticides are used to contain anthracnose disease with limited success. In this study, we assessed the potential of Host-Induced Gene Silencing (HIGS) approach to target the Colletotrichum gloeosporioides COM1 (CgCOM1) developmental gene involved in the fungal conidial and appressorium formation, to restrict fungal infection in chilli and tomato fruits. For this study, we have developed stable transgenic lines of chilli and tomato expressing CgCOM1-RNAi construct employing Agrobacterium-mediated transformation. Transgenic plants were characterized by molecular and gene expression analyses. Production of specific CgCOM1 siRNA in transgenic chilli and tomato RNAi lines was confirmed by stem-loop RT-PCR. Fungal challenge assays on leaves and fruits showed that the transgenic lines were resistant to anthracnose disease-causing C. gloeosporioides in comparison to wild type and empty-vector control plants. RT-qPCR analyses in transgenic lines revealed extremely low abundance of CgCOM1 transcripts in the C. gloeosporioides infected tissues, indicating near complete silencing of CgCOM1 gene expression in the pathogen. Microscopic examination of the Cg-challenged leaves of chilli-CgCOM1i lines revealed highly suppressed conidial germination, germ tube development, appressoria formation and mycelial growth of C. gloeosporioides, resulting in reduced infection of plant tissues. These results demonstrated highly efficient use of HIGS in silencing the expression of essential fungal developmental genes to inhibit the growth of pathogenic fungi, thus providing a highly precise approach to arrest the spread of disease.
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