Evidence map›Paper›PMID 32802193›Full record

ArticleTheranostics2020

Nanozyme-assisted sensitive profiling of exosomal proteins for rapid cancer diagnosis.

Huixia Di, Ze Mi, Yan Sun, Xuehui Liu, Xinzhuo Liu, Ang Li, Ying Jiang, Hongmei Gao, Pengfei Rong, Dingbin Liu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Theranostics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.

0numbers the graph read from it
0cells of the map it votes in
30citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

30 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Huixia DiResearch Center for Analytical Sciences in the College of Chemistry, Tianjin First Central Hospital, State Key Laboratory of Medicinal Chemical Biology, and Tianjin Key Laboratory of Molecular Recognition and Biosensing, Nankai University, Tianjin 300071, China.
Ze MiDepartment of Radiology, The Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, China.
Yan SunResearch Center for Analytical Sciences in the College of Chemistry, Tianjin First Central Hospital, State Key Laboratory of Medicinal Chemical Biology, and Tianjin Key Laboratory of Molecular Recognition and Biosensing, Nankai University, Tianjin 300071, China.
Xuehui LiuResearch Center for Analytical Sciences in the College of Chemistry, Tianjin First Central Hospital, State Key Laboratory of Medicinal Chemical Biology, and Tianjin Key Laboratory of Molecular Recognition and Biosensing, Nankai University, Tianjin 300071, China.
Xinzhuo LiuResearch Center for Analytical Sciences in the College of Chemistry, Tianjin First Central Hospital, State Key Laboratory of Medicinal Chemical Biology, and Tianjin Key Laboratory of Molecular Recognition and Biosensing, Nankai University, Tianjin 300071, China.
Ang LiResearch Center for Analytical Sciences in the College of Chemistry, Tianjin First Central Hospital, State Key Laboratory of Medicinal Chemical Biology, and Tianjin Key Laboratory of Molecular Recognition and Biosensing, Nankai University, Tianjin 300071, China.
Ying JiangResearch Center for Analytical Sciences in the College of Chemistry, Tianjin First Central Hospital, State Key Laboratory of Medicinal Chemical Biology, and Tianjin Key Laboratory of Molecular Recognition and Biosensing, Nankai University, Tianjin 300071, China.
Hongmei GaoResearch Center for Analytical Sciences in the College of Chemistry, Tianjin First Central Hospital, State Key Laboratory of Medicinal Chemical Biology, and Tianjin Key Laboratory of Molecular Recognition and Biosensing, Nankai University, Tianjin 300071, China.
Pengfei RongDepartment of Radiology, The Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, China.
Dingbin LiuResearch Center for Analytical Sciences in the College of Chemistry, Tianjin First Central Hospital, State Key Laboratory of Medicinal Chemical Biology, and Tianjin Key Laboratory of Molecular Recognition and Biosensing, Nankai University, Tianjin 300071, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The proteins expressed on exosomes have emerged as promising liquid-biopsy biomarkers for cancer diagnosis. However, molecular profiling of exosomal proteins remains technically challenging. Herein, we report a nanozyme-assisted immunosorbent assay (NAISA) that enables sensitive and rapid multiplex profiling of exosomal proteins. This NAISA system is based on the installation of peroxidase-like nanozymes onto the phospholipid membranes of exosomes, thus avoiding the need for post-labelling detection antibodies. The exosomal proteins are determined by a sensitive nanozyme-catalyzed colorimetric assay less than 3 h, without the need for multi-step incubation and washing operations. Using NAISA to profile exosomal proteins from different cell lines and clinical samples, we reveal that tumor-associated exosomal proteins can serve as promising biomarkers for accurate cancer diagnosis in a cooperative detection pattern.

Indexed as

BenzidinesBiomarkers, TumorCarcinoma, HepatocellularCell Line, TumorColorimetryExosomesGoldHep G2 CellsHumansLiver NeoplasmsMetal NanoparticlesNeoplasm ProteinsPeroxidase3,3',5,5'-tetramethylbenzidineBenzidinesBiomarkers, TumorGoldNeoplasm ProteinsPeroxidasecancer diagnosisExosomenanozymenanozyme-assisted immunosorbent assayprotein profiling

Identifiers

PMID32802193
PMCPMC7415820

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.