Evidence map›Paper›PMID 32797667›Full record

ArticleAngewandte Chemie (International ed. in English)2020

A Genetically Encoded RNA Photosensitizer for Targeted Cell Regulation.

Kewei Ren, Puspam Keshri, Rigumula Wu, Zhining Sun, Qikun Yu, Qian Tian, Bin Zhao, Yousef Bagheri, Yiwen Xie, Mingxu You

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Angewandte Chemie (International ed. in English), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 53% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Kewei RenDepartment of Chemistry, University of Massachusetts, Amherst, MA, 01003, USA.
Puspam KeshriDepartment of Chemistry, University of Massachusetts, Amherst, MA, 01003, USA.
Rigumula WuDepartment of Chemistry, University of Massachusetts, Amherst, MA, 01003, USA.
Zhining SunDepartment of Chemistry, University of Massachusetts, Amherst, MA, 01003, USA.
Qikun YuDepartment of Chemistry, University of Massachusetts, Amherst, MA, 01003, USA.
Qian TianDepartment of Chemistry, University of Massachusetts, Amherst, MA, 01003, USA.
Bin ZhaoDepartment of Chemistry, University of Massachusetts, Amherst, MA, 01003, USA.
Yousef BagheriDepartment of Chemistry, University of Massachusetts, Amherst, MA, 01003, USA.
Yiwen XieDepartment of Chemistry, University of Massachusetts, Amherst, MA, 01003, USA.
Mingxu YouDepartment of Chemistry, University of Massachusetts, Amherst, MA, 01003, USA.ORCID 0000-0002-5279-2340
University of Massachusetts Amherst · US

Funding

Genetically-encoded fluorescent RNA sensors for measuring transport of antibiotics into the cytoplasm of Gram-negative pathogens and development of efflux pump inhibitorsR01AI136789 · NIAID · HARVARD MEDICAL SCHOOL · PI LORY, STEPHEN · 2018 to 2022
$3.6M
NIAID NIH HHS R01 AI136789
6 · The paper itself

Abstract

Genetically encoded RNA devices have emerged for various cellular applications in imaging and biosensing, but their functions as precise regulators in living systems are still limited. Inspired by protein photosensitizers, we propose here a genetically encoded RNA aptamer based photosensitizer (GRAP). Upon illumination, the RNA photosensitizer can controllably generate reactive oxygen species for targeted cell regulation. The GRAP system can be selectively activated by endogenous stimuli and light of different wavelengths. Compared with their protein analogues, GRAP is highly programmable and exhibits reduced off-target effects. These results indicate that GRAP enables efficient noninvasive target cell ablation with high temporal and spatial precision. This new RNA regulator system will be widely used for optogenetics, targeted cell ablation, subcellular manipulation, and imaging.

Indexed as

Aptamers, NucleotideEscherichia coliHeLa CellsHumansOptical ImagingPhotosensitizing AgentsReactive Oxygen SpeciesAptamers, NucleotidePhotosensitizing AgentsReactive Oxygen Speciesaptamersimaging agentsphotosensitizersreactive oxygen speciesRNA

Identifiers

PMID32797667
PMCPMC7747015
OpenAlexW3049311202

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.