ArticleMethods in molecular biology (Clifton, N.J.)2021
Preservation of Mammalian Sperm by Freeze-Drying.
Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- In vitro effects of green tea (Camellia sinensis) aqueous extract on sperm quality: Mitigating oxidative stress and sperm DNA fragmentation in freeze-dried human spermatozoa.Clinical and experimental reproductive medicine · 2026Article
- Reviving vacuum-dried encapsulated ram spermatozoa via ICSI after 2 years of storage.Frontiers in veterinary science · 2023Article
- Long-term preservation of germ cells and gonadal tissues at ambient temperatures.Reproduction & fertility · 2022Review
- Long-term storage of gametes and gonadal tissues at room temperatures: the end of the ice age?Journal of assisted reproduction and genetics · 2022Article
- Desiccated cat spermatozoa retain DNA integrity and developmental potential after prolonged storage and shipping at non-cryogenic temperatures.Journal of assisted reproduction and genetics · 2022Article
- Drying and temperature induced conformational changes of nucleic acids and stallion sperm chromatin in trehalose preservation formulations.Scientific reports · 2021Article
- Update on techniques for cryopreservation of human spermatozoa.Asian journal of andrologyReview
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Long-term preservation of mammalian sperm at suprazero temperatures is desired to save storage and space costs, as well as to facilitate transport of preserved samples. This can be accomplished by the freeze-drying of sperm samples. Although freeze-drying results in immotile and membrane-compromised sperm, intracytoplasmic sperm injection (ICSI) can be used to introduce such an immotile sperm into an oocyte and thus start the fertilization process. So far, it has been shown that improved freeze-drying protocols preserve chromosomal integrity and oocyte-activating factor(s) in rodent and mammalian species at 4 °C for several years and at ambient temperature for up to 1 year depending on species, which permits shipping freeze-dried samples at ambient temperature. This chapter concisely reviews freeze-drying of mammalian sperm first and then presents a simple freeze-drying protocol.
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Registered trials
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