Evidence map›Paper›PMID 32797420›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2021

Raman Cryomicroscopic Imaging and Sample Holder for Spectroscopic Subzero Temperature Measurements.

Guanglin Yu, Rui Li, Allison Hubel

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
4.4field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Guanglin YuDepartment of Mechanical Engineering, University of Minnesota, Minneapolis, MN, USA.
Rui LiDepartment of Biomedical Engineering, University of Minnesota, Minneapolis, MN, USA.
Allison HubelDepartment of Mechanical Engineering, University of Minnesota, Minneapolis, MN, USA. hubel001@umn.edu.
University of Minnesota · US

Funding

Multicomponent solutions for the preservation of cell therapy productsR01EB023880 · NIBIB · UNIVERSITY OF MINNESOTA · PI HUBEL, ALLISON · 2017 to 2019
$1.1M
NIBIB NIH HHS R01 EB023880
6 · The paper itself

Abstract

Raman spectroscopy has been gaining in popularity for noninvasive analysis of single cells. Raman spectra and images deliver meaningful information regarding the biochemical, biophysical, and structural properties of cells in various states. Low-temperature Raman spectroscopy has been applied to verify the presence of ice inside a frozen cell and to illustrate the distribution of both penetrating and non-penetrating cryoprotectants. This chapter delineates Raman cryomicroscopic imaging of single cells as well as sample handling for spectroscopic measurements at subzero temperature. The experimental setup is depicted with a special emphasis on a custom-built temperature-controlled cooling stage. The use of Raman cryomicroscopic imaging is demonstrated using Jurkat cells cryopreserved in a sucrose solution. Moreover, strategies for determining intracellular ice formation (IIF) and analysis of sucrose partitioning across the cell membrane are presented.

Indexed as

Cold TemperatureCell MembraneCryopreservationCryoprotective AgentsFreeze DryingHumansIceImage Processing, Computer-AssistedJurkat CellsPhase TransitionSpectrum Analysis, RamanCryoprotective AgentsIceCellCryopreservationHyperspectral imagingLow-temperature microscopyRaman spectroscopy

Identifiers

PMID32797420
PMCPMC8214853
OpenAlexW3049627486

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.