ArticleMethods in molecular biology (Clifton, N.J.)2021
Principles Underlying Cryopreservation and Freeze-Drying of Cells and Tissues.
Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- In vitro effects of green tea (Camellia sinensis) aqueous extract on sperm quality: Mitigating oxidative stress and sperm DNA fragmentation in freeze-dried human spermatozoa.Clinical and experimental reproductive medicine · 2026Article
- Low-molecular-weight polysaccharide and polyol impregnation enhances the rehydration recovery capacity of freeze-dried potato slices.Food chemistry: X · 2026Article
- Whole testicular organ and seminal cryopreservation in the malarial mosquito Anopheles gambiae (Diptera: Culicidae).Journal of medical entomology · 2026Article
- Serum-derived albumin as a cryoprotective agent for the long-term storage of red blood cells: A preliminary examination.Transfusion · 2026Article
- Application of Freeze-Drying Technology in the Food Industry: A Review.Foods (Basel, Switzerland) · 2026Review
- Drying parameters and aging modulate protective properties of vitrified trehalose.bioRxiv : the preprint server for biology · 2026Article
- Drying parameters and aging modulate protective properties of vitrified trehalose.BBA advances · 2026Article
- Characteristics of cell adhesion molecules expression and environmental adaptation in yak lung tissue.Scientific reports · 2025Article
- Overview of Glycometabolism of Lactic Acid Bacteria During Freeze-Drying: Changes, Influencing Factors, and Application Strategies.Foods (Basel, Switzerland) · 2025Review
- Successful cryopreservation of marine invertebrates immune cells enables long-term studies of common octopus,Frontiers in immunology · 2025Article
- From tradition to trait: stay-green alleles in SudaneseFrontiers in plant science · 2025Article
- Effectiveness of Lyoprotectants in Protein Stabilization During Lyophilization.Pharmaceutics · 2024Review
- Optimisation of cryopreservation conditions, including storage duration and revival methods, for the viability of human primary cells.BMC molecular and cell biology · 2024Article
- Ice formation and its elimination in cryopreservation of oocytes.Scientific reports · 2024Article
- Monarch butterfly conservation through male germplasm cryopreservation.Scientific reports · 2024Article
- Investigation of the Solid-State Interactions in Lyophilized Human G-CSF Using Hydrogen-Deuterium Exchange Mass Spectrometry.Molecular pharmaceutics · 2024Article
- The Journal of law, medicine & ethics : a journal of the American Society of Law, Medicine & Ethics · 2024Article
- Slow Freezing of Preserved Boar Sperm: Comparison of Conventional and Automated Techniques on Post-Thaw Functional Quality by a New Combination of Sperm Function Tests.Animals : an open access journal from MDPI · 2023Article
- Preparation and assessment of an optimized multichannel acellular nerve allograft for peripheral nerve regeneration.Bioengineering & translational medicine · 2023Article
- Effect of Process Parameters, Protectants and Carrier Materials on the Survival of Yeast Cells during Fluidized Bed Granulation for Tableting.Pharmaceutics · 2023Article
Corrections and comments
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Authors and funding
2 authors.
Funding
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Abstract
Cryopreservation and freeze-drying can be used to preserve cells or tissues for prolonged periods. Vitrification, or ice-free cryopreservation, is an alternative to cryopreservation that enables cooling cells to cryogenic temperatures in the absence of ice. The processing pathways involved in (ice-free) cryopreservation and freeze-drying of cells and tissues, however, can be very damaging. In this chapter, we describe the principles underlying preservation of cells for which freezing and drying are normally lethal processes as well as for cells that are able to survive in a reversible state of suspended animation. Freezing results in solution effects injury and/or intracellular ice formation, whereas drying results in removal of (non-freezable) water normally bound to biomolecules, which is generally more damaging. Cryopreservation and freeze-drying require different types of protective agents. Different mechanistic modes of action of cryoprotective and lyoprotective agents are described including minimizing ice formation, preferential exclusion, water replacement, and vitrification. Furthermore, it is discussed how protective agents can be introduced into cells avoiding damage due to too large cell volume excursions, and how knowledge of cell-specific membrane permeability properties in various temperature regimes can be used to rationally design (ice-free) cryopreservation and freeze-drying protocols.
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