Evidence map›Paper›PMID 32796744›Full record

ArticleInternational journal of molecular sciences2020

Exploring the Biochemical Origin of DNA Sequence Variation in Barley Plants Regenerated via in Vitro Anther Culture.

Piotr T Bednarek, Jacek Zebrowski, Renata Orłowska

Open access · goldAbstract read
In one paragraph

Article in International journal of molecular sciences, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
1.1field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 18 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Review
  8. Article
  9. Article
  10. Article
  11. Article
  12. Review
  13. Article
  14. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Piotr T BednarekDepartment of Plant Physiology and Biochemistry, Plant Breeding and Acclimatization Institute-National Research Institute, Radzików, 05-870 Błonie, Poland.
Jacek ZebrowskiInstitute of Biology and Biotechnology, University of Rzeszow, Al. Rejtana 16c A, 35-959 Rzeszow, Poland.ORCID 0000-0001-8424-857X
Renata OrłowskaDepartment of Plant Physiology and Biochemistry, Plant Breeding and Acclimatization Institute-National Research Institute, Radzików, 05-870 Błonie, Poland.ORCID 0000-0001-7150-6920
Plant Breeding and Acclimatization Institute - National Research Institute · PLUniversity of Rzeszów · PL

Funding

MINISTRY OF AGRICULTURE AND RURAL DEVELOPMENT (Poland) HORhn-801-PB-22/15-18
6 · The paper itself

Abstract

Tissue culture is an essential tool for the regeneration of uniform plant material. However, tissue culture conditions can be a source of abiotic stress for plants, leading to changes in the DNA sequence and methylation patterns. Despite the growing evidence on biochemical processes affected by abiotic stresses, how these altered biochemical processes affect DNA sequence and methylation patterns remains largely unknown. In this study, the methylation-sensitive Amplified Fragment Length Polymorphism (metAFLP) approach was used to investigate de novo methylation, demethylation, and sequence variation in barley regenerants derived by anther culture. Additionally, we used Attenuated Total Reflectance Fourier Transform Infrared (ATR-FTIR) spectroscopy to identify the spectral features of regenerants, which were then analyzed by mediation analysis. The infrared spectrum ranges (710-690 and 1010-940 cm

Indexed as

Genetic VariationRegenerationTissue Culture TechniquesAmplified Fragment Length Polymorphism AnalysisBase SequenceDemethylationFlowersHordeumSpectroscopy, Fourier Transform InfraredATR-FTIR spectroscopycelluloseDNA demethylationS-adenosyl L-methioninesequence variationtime of in vitro cultureβ-glucans

Identifiers

PMID32796744
PMCPMC7461140
OpenAlexW3048753295

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.