ArticleInternational journal of molecular sciences2020
Enhanced Heterologous Production of Glycosyltransferase UGT76G1 by Co-Expression of Endogenous
Article in International journal of molecular sciences, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 30 citations in OpenAlex.
- Dual-Enzyme Biocatalytic Synthesis of Novel Rebaudioside M Analogs with Enhanced Aqueous Solubility, Physicochemical Stability, and Sensory Profiles.Foods (Basel, Switzerland) · 2026Article
- Discovery and engineering of UDP-glycosyltransferase and its applications in natural sweetener glycosylation.World journal of microbiology & biotechnology · 2026Review
- A Comprehensive Review on Steviol Glycosides: Sources, Properties, Bioactivities, Sensory-Functional Enhancement and Bioproduction Strategies.Plants (Basel, Switzerland) · 2026Review
- The progress on stevia (Frontiers in nutrition · 2026Review
- Enhanced Production of Rebaudioside D and Rebaudioside M through V155T Substitution in the Glycosyltransferase UGT91D2 fromJournal of agricultural and food chemistry · 2025Article
- Discovery, characterization, and comparative analysis of new UGT72 and UGT84 family glycosyltransferases.Communications chemistry · 2024Article
- A novel starch-active lytic polysaccharide monooxygenase discovered with bioinformatics screening and its application in textile desizing.BMC biotechnology · 2024Article
- Recent Advances in the Biosynthesis of Natural Sugar Substitutes in Yeast.Journal of fungi (Basel, Switzerland) · 2023Review
- Trehalose Production Using Three Extracellular Enzymes Produced via One-Step Fermentation of an EngineeredBioengineering (Basel, Switzerland) · 2023Article
- Rethinking Biosynthesis of Aclacinomycin A.Molecules (Basel, Switzerland) · 2023Review
- De novo biosynthesis of rubusoside and rebaudiosides in engineered yeasts.Nature communications · 2022Article
- Highly efficient production of rebaudioside D enabled by structure-guided engineering of bacterial glycosyltransferase YojK.Frontiers in bioengineering and biotechnology · 2022Article
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
Abstract
Steviol glycosides (SGs) with zero calories and high-intensity sweetness are the best substitutes of sugar for the human diet. Uridine diphosphate dependent glycosyltransferase (UGT) UGT76G1, as a key enzyme for the biosynthesis of SGs with a low heterologous expression level, hinders its application. In this study, a suitable fusion partner, Smt3, was found to enhance the soluble expression of UGT76G1 by 60%. Additionally, a novel strategy to improve the expression of Smt3-UGT76G1 was performed, which co-expressed endogenous genes
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.