Evidence map›Paper›PMID 32795399›Full record

ArticleCell stem cell2020

Naive Pluripotent Stem Cells Exhibit Phenotypic Variability that Is Driven by Genetic Variation.

Daniel Ortmann, Stephanie Brown, Anne Czechanski, Selcan Aydin, Daniele Muraro, Yuanhua Huang, Rute A Tomaz, Anna Osnato, Giovanni Canu, Brandon T Wesley and 9 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell stem cell, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.

0numbers the graph read from it
0cells of the map it votes in
35citing papers in PubMed
3.2field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

35 citing papers in PubMed, 55 citations in OpenAlex.

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  15. Gastruloid optimization.Emerging topics in life sciences · 2023
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

19 authors at 4 institutions in 3 countries.

Daniel OrtmannWellcome Trust and MRC Cambridge Stem Cell Institute, University of Cambridge, Cambridge, UK; Department of Surgery, University of Cambridge, Cambridge, UK. Electronic address: daniel.ortmann@gmail.com.
Stephanie BrownWellcome Trust and MRC Cambridge Stem Cell Institute, University of Cambridge, Cambridge, UK; Department of Surgery, University of Cambridge, Cambridge, UK.
Anne CzechanskiJackson Laboratory, Bar Harbor, ME, USA.
Selcan AydinJackson Laboratory, Bar Harbor, ME, USA.
Daniele MuraroWellcome Trust and MRC Cambridge Stem Cell Institute, University of Cambridge, Cambridge, UK; Department of Surgery, University of Cambridge, Cambridge, UK; Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, UK.
Yuanhua HuangDepartment of Clinical Neurosciences, University of Cambridge, Cambridge, UK; European Molecular Biology Laboratory, European Bioinformatics Institute, Wellcome Genome Campus, Hinxton, UK.
Rute A TomazWellcome Trust and MRC Cambridge Stem Cell Institute, University of Cambridge, Cambridge, UK; Department of Surgery, University of Cambridge, Cambridge, UK.
Anna OsnatoWellcome Trust and MRC Cambridge Stem Cell Institute, University of Cambridge, Cambridge, UK; Department of Surgery, University of Cambridge, Cambridge, UK.
Giovanni CanuWellcome Trust and MRC Cambridge Stem Cell Institute, University of Cambridge, Cambridge, UK; Department of Surgery, University of Cambridge, Cambridge, UK.
Brandon T WesleyWellcome Trust and MRC Cambridge Stem Cell Institute, University of Cambridge, Cambridge, UK; Department of Surgery, University of Cambridge, Cambridge, UK.
Daniel A SkellyJackson Laboratory, Bar Harbor, ME, USA.
Oliver StegleEuropean Molecular Biology Laboratory, European Bioinformatics Institute, Wellcome Genome Campus, Hinxton, UK; European Molecular Biology Laboratory, Genome Biology Unit, Heidelberg, Germany; Division of Computational Genomics and Systems Genetics, German Cancer Research, Center (DKFZ), Heidelberg, Germany.
Ted ChoiJackson Laboratory, Bar Harbor, ME, USA.
Gary A ChurchillJackson Laboratory, Bar Harbor, ME, USA.
Christopher L BakerJackson Laboratory, Bar Harbor, ME, USA.
Peter J Rugg-GunnWellcome Trust and MRC Cambridge Stem Cell Institute, University of Cambridge, Cambridge, UK; Epigenetics Programme, Babraham Institute, Cambridge, UK.
Steven C MungerJackson Laboratory, Bar Harbor, ME, USA.
Laura G ReinholdtJackson Laboratory, Bar Harbor, ME, USA.
Ludovic VallierWellcome Trust and MRC Cambridge Stem Cell Institute, University of Cambridge, Cambridge, UK; Department of Surgery, University of Cambridge, Cambridge, UK. Electronic address: lv225@cam.ac.uk.
Jackson Laboratory · USWellcome/MRC Cambridge Stem Cell Institute · GBEuropean Bioinformatics Institute · GBBabraham Institute · GB

Funding

The Mutant Mouse Resource and Research Center at The Jackson LaboratoryU42OD010921 · OD · JACKSON LABORATORY · PI LAURA G REINHOLDT · 2012 to 2026
$21.4M
Use for ResourceP40OD011102 · OD · JACKSON LABORATORY · PI Steven Carmen Munger, Stephen A Murray · 2012 to 2026
$15.0M
Leveraging Natural Genetic Diversity and Systems Genetics to Elucidate the Complex Hierarchy of Gene Regulation Underlying Ground State Pluripotency, Cell Fate Decisions and Tissue HomeostasisR35GM133495 · NIGMS · JACKSON LABORATORY · PI MUNGER, STEVEN CARMEN · 2019 to 2023
$2.0M
Medical Research Council G0701448Medical Research Council MC_PC_12009Medical Research Council MC_PC_17230National Centre for the Replacement, Refinement and Reduction of Animals in Research NC/N001540/1National Centre for the Replacement, Refinement and Reduction of Animals in Research NC/T2T0419NIGMS NIH HHS R35 GM133495NIH HHS P40 OD011102NIH HHS U42 OD010921Wellcome Trust
6 · The paper itself

Abstract

Variability among pluripotent stem cell (PSC) lines is a prevailing issue that hampers not only experimental reproducibility but also large-scale applications and personalized cell-based therapy. This variability could result from epigenetic and genetic factors that influence stem cell behavior. Naive culture conditions minimize epigenetic fluctuation, potentially overcoming differences in PSC line differentiation potential. Here we derived PSCs from distinct mouse strains under naive conditions and show that lines from distinct genetic backgrounds have divergent differentiation capacity, confirming a major role for genetics in PSC phenotypic variability. This is explained in part through inconsistent activity of extra-cellular signaling, including the Wnt pathway, which is modulated by specific genetic variants. Overall, this study shows that genetic background plays a dominant role in driving phenotypic variability of PSCs.

Indexed as

Induced Pluripotent Stem CellsPluripotent Stem CellsAnimalsBiological Variation, PopulationCell DifferentiationGenetic VariationMiceReproducibility of ResultsdifferentiationeQTLgeneticsground statemouse embryonic stem cellsnaïvepluripotent stem cellssignalingvariability

Identifiers

PMID32795399
PMCPMC7487768
OpenAlexW3048813231

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.