Evidence map›Paper›PMID 32794261›Full record

ArticleExperimental dermatology2020

Inducible nitric oxide synthase is required for epidermal permeability barrier homeostasis in mice.

Erle Dang, George Man, Jiechen Zhang, Dale Lee, Theodora M Mauro, Peter M Elias, Mao-Qiang Man

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Experimental dermatology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.6field-weighted citation impact, top 40% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 16 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
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  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Erle DangDermatology Service Veterans Affairs Medical Center, Department of Dermatology, University of California, San Francisco, CA, USA.
George ManDermatology Service Veterans Affairs Medical Center, Department of Dermatology, University of California, San Francisco, CA, USA.
Jiechen ZhangDermatology Service Veterans Affairs Medical Center, Department of Dermatology, University of California, San Francisco, CA, USA.
Dale LeeDermatology Service Veterans Affairs Medical Center, Department of Dermatology, University of California, San Francisco, CA, USA.
Theodora M MauroDermatology Service Veterans Affairs Medical Center, Department of Dermatology, University of California, San Francisco, CA, USA.
Peter M EliasDermatology Service Veterans Affairs Medical Center, Department of Dermatology, University of California, San Francisco, CA, USA.
Mao-Qiang ManDermatology Service Veterans Affairs Medical Center, Department of Dermatology, University of California, San Francisco, CA, USA.ORCID 0000-0002-0957-4903
San Francisco VA Medical Center · US

Funding

Pathogenesis and Therapy of Ichthyosis in Disorders of Lipid MetabolismR01AR061106 · NIAMS · NORTHERN CALIFORNIA INSTITUTE/RES/EDU · PI MAURO, THEODORA M · 2012 to 2022
$3.3M
NIAMS NIH HHS AR061106NIAMS NIH HHS R01 AR061106
6 · The paper itself

Abstract

Nitric oxide (NO) regulates a variety of epidermal functions, including epidermal proliferation, differentiation and cutaneous wound healing. However, whether nitric oxide (NO) and its synthetic enzymes regulate epidermal permeability barrier homeostasis is not clear. In the present study, we employed inducible nitric oxide synthase (iNOS) KO mice to explore the role of iNOS in epidermal permeability barrier homeostasis. Our results showed that iNOS mice displayed a comparable levels of basal transepidermal water loss rates, stratum corneum hydration and skin surface pH to their wild-type mice, but epidermal permeability barrier recovery was significantly delayed both 2 and 4 hours after acute barrier disruption by tape stripping. In parallel, expression levels of mRNA for epidermal differentiation-related proteins and lipid synthetic enzymes were lower in iNOS KO mice versus wild-type controls. Topical applications of two structurally unrelated NO donors to iNOS KO mice improved permeability barrier recovery kinetics and upregulated expression levels of mRNA for epidermal differentiation-related proteins and lipid synthetic enzymes. Together, these results indicate that iNOS and its product regulate epidermal permeability barrier homeostasis in mice.

Indexed as

HomeostasisAnimalsCell DifferentiationEpidermisFilaggrin ProteinsGene ExpressionHydrogen-Ion ConcentrationIntermediate Filament ProteinsKeratinocytesLipid MetabolismMembrane ProteinsMiceMice, KnockoutNitric OxideNitric Oxide DonorsNitric Oxide Synthase Type IIFilaggrin ProteinsIntermediate Filament ProteinsinvolucrinloricrinMembrane ProteinsNitric OxideNitric Oxide DonorsNitric Oxide Synthase Type IINos2 protein, mouseProtein PrecursorsRNA, MessengerS-Nitroso-N-Acetylpenicillamine

Identifiers

PMID32794261
PMCPMC8754083
OpenAlexW3049187743

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.