Evidence map›Paper›PMID 32793925›Full record

ArticlemedRxiv : the preprint server for health sciences2020

Comparison of media and standards for SARS-CoV-2 RT-qPCR without prior RNA preparation.

Katherine B Ragan, Sanchita Bhadra, Joon H Choi, Dalton Towers, Christopher S Sullivan, Andrew D Ellington

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in medRxiv : the preprint server for health sciences, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Katherine B RaganDepartment of Molecular Biosciences, College of Natural Sciences, The University of Texas at Austin, Austin, TX 78712, USA.
Sanchita BhadraDepartment of Molecular Biosciences, College of Natural Sciences, The University of Texas at Austin, Austin, TX 78712, USA.
Joon H ChoiDepartment of Molecular Biosciences, College of Natural Sciences, The University of Texas at Austin, Austin, TX 78712, USA.
Dalton TowersDepartment of Molecular Biosciences, College of Natural Sciences, The University of Texas at Austin, Austin, TX 78712, USA.
Christopher S SullivanDepartment of Molecular Biosciences, College of Natural Sciences, The University of Texas at Austin, Austin, TX 78712, USA.
Andrew D EllingtonDepartment of Molecular Biosciences, College of Natural Sciences, The University of Texas at Austin, Austin, TX 78712, USA.

Funding

Non-coding RNA and ADP-ribosylation in Antiviral DefenseR01AI123231 · NIAID · UNIVERSITY OF TEXAS AT AUSTIN · PI SULLIVAN, CHRISTOPHER S. · 2017 to 2021
$1.9M
Elucidating How Tri-phosphatase DUSP11 Controls HCV Infection and Hepatocyte InflammationR01AI134980 · NIAID · UNIVERSITY OF TEXAS AT AUSTIN · PI RANDALL, GLENN C, SULLIVAN, CHRISTOPHER S. · 2018 to 2021
$1.8M
Directed evolution of polymerases that can read and write extremely long sequencesR01EB027202 · NIBIB · UNIVERSITY OF TEXAS AT AUSTIN · PI ELLINGTON, ANDREW D · 2020 to 2024
$1.6M
NIAID NIH HHS R01 AI123231NIAID NIH HHS R01 AI134980NIBIB NIH HHS R01 EB027202
6 · The paper itself

Abstract

Since the emergence of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic, there have been demands on the testing infrastructure that have strained testing capacity. As a simplification of method, we confirm the efficacy of RNA extraction-free RT-qPCR and saline as an alternative patient sample storage buffer. In addition, amongst potential reagent shortages, it has sometimes been difficult to obtain inactivated viral particles. We have therefore also characterized armored SARS-CoV-2 RNA from Asuragen as an alternative diagnostic standard to ATCC genomic SARS-CoV-2 RNA and heat inactivated virions and provide guidelines for its use in RT-qPCR.

Identifiers

PMID32793925
PMCPMC7418746

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.