ArticleNucleic acids research1988
A heat shock element in the phosphoglycerate kinase gene promoter of yeast.
Article in Nucleic acids research, 1988. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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21 citing papers in PubMed, 43 citations in OpenAlex.
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- Identification of a Heat-Inducible Element of Cysteine Desulfurase Gene Promoter inMolecules (Basel, Switzerland) · 2019Article
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- MAPKKK-independent regulation of the Hog1 stress-activated protein kinase in Candida albicans.The Journal of biological chemistry · 2011Article
- Microarray based analysis of temperature and oxidative stress induced messenger RNA in Schistosoma mansoni.Molecular and biochemical parasitology · 2008Article
- Activity of the plasma membrane H(+)-ATPase and optimal glycolytic flux are required for rapid adaptation and growth of Saccharomyces cerevisiae in the presence of the weak-acid preservative sorbic acid.Applied and environmental microbiology · 1996Article
- The 3-phosphoglycerate kinase gene of the yeast Yarrowia lipolytica de-represses on gluconeogenic substrates.Current genetics · 1996Article
- Expression and secretion of recombinant ovine beta-lactoglobulin in Saccharomyces cerevisiae and Kluyveromyces lactis.The Biochemical journal · 1996Article
- Cloning and characterization of seven cDNAs for hyperosmolarity-responsive (HOR) genes of Saccharomyces cerevisiae.Molecular & general genetics : MGG · 1995Article
- Characterisation of Saccharomyces cerevisiae genes encoding ribosomal protein YL6.Molecular & general genetics : MGG · 1995Article
- Characterization of uvi15+, a stress-inducible gene from Schizosaccharomyces pombe.Molecular & general genetics : MGG · 1995Article
- The yeast and mammalian Ras pathways control transcription of heat shock genes independently of heat shock transcription factor.Molecular and cellular biology · 1994Article
- The yeast co-activator GAL11 positively influences transcription of the phosphoglycerate kinase gene, but only when RAP1 is bound to its upstream activation sequence.Molecular & general genetics : MGG · 1994Article
- Stress response of yeast.The Biochemical journal · 1993Review
- Studies on plasmid stability, cell metabolism and superoxide dismutase production by Pgk- strains of Saccharomyces cerevisiae.Applied microbiology and biotechnology · 1992Article
- Yeast glycolytic mRNAs are differentially regulated.Molecular and cellular biology · 1991Article
- Interdependence of several heat shock gene activations, cyclic AMP decline and changes at the plasma membrane of Saccharomyces cerevisiae.Antonie van Leeuwenhoek · 1990Review
- Multiple factors bind the upstream activation sites of the yeast enolase genes ENO1 and ENO2: ABFI protein, like repressor activator protein RAP1, binds cis-acting sequences which modulate repression or activation of transcription.Molecular and cellular biology · 1990Article
- When a glycolytic gene on a yeast 2 mu ORI-STB plasmid is made essential for growth its expression level is a major determinant of plasmid copy number.Current genetics · 1990Article
- Isolation and characterization of STI1, a stress-inducible gene from Saccharomyces cerevisiae.Molecular and cellular biology · 1989Article
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The phosphoglycerate kinase (PGK) promoter is often employed in yeast expression vectors due to its very high efficiency. Its activity in unstressed cells has been shown to be due to an upstream activator site (UASPGK) at -402 to -479. Since levels of PGK mRNA can sometimes be elevated by heat shock of yeast cultures this investigation determined how specific deletions of PGK promoter sequences effect levels of PGK mRNA both before and after heat shock. A series of PGK promoter deletions was inserted on a high copy plasmid into cells having a TRP1 gene disruption of the solitary chromosomal PGK locus. This enabled PGK transcripts of plasmid and chromosomal origin to be distinguished by virtue of their different sizes. Certain deletions lacking UASPGK displayed activities that were very low in unstressed cells, but which increased fifty to one-hundred fold after heat shock. With UASPGK present heat shock had only a relatively small or negligible effect on PGK mRNA levels. Heat shock activation was abolished when the -256 to -377 region with homology to the heat shock element consensus of eukaryotes was deleted in addition to UASPGK, but was unaffected by the deletion of regions further downstream containing TATA- and CAAT- sequence motifs. This is the first demonstration of a heat shock element, an activator site normally found upstream of eukaryotic heat shock protein genes, as a natural constituent of a high efficiency glycolytic promoter. It is proposed that PGK may be one member of a small subset of yeast genes that are highly expressed in unstressed cells yet possess a heat shock element to ensure their continued transcription after heat shock.
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