ArticleAoB PLANTS2020
Direct fluorescence imaging of lignocellulosic and suberized cell walls in roots and stems.
Article in AoB PLANTS, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 68 citations in OpenAlex.
- Canonical Ru(ii) tris-polypyridyl complexes as confocal-compatible stains for lignified secondary-wall domains inRSC advances · 2026Article
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- Visualizing Dynamic Interfacial Assembly in Pickering Emulsions with Fluorescent Cellulose Nanofibers.The journal of physical chemistry letters · 2026Article
- The lignin fluorescence at the base of the petals guiding the pollinators to the pollination chamber in Artabotrys hainanensis.BMC plant biology · 2026Article
- Plant Microtechnique with Resin: Towards Plant Histolomics.Plants (Basel, Switzerland) · 2026Article
- Chemical Composition and Structural Traits of Leaf Biomass in Selected Asparagaceae Species.Plants (Basel, Switzerland) · 2026Article
- Study of Hemp Fiber Properties Modified via Long-Duration Low-Pressure Argon and Oxygen Plasma Treatments.ACS omega · 2025Article
- Cell wall remodeling and polarized light analysis reveal ecotype-specific strategies in Salicornia europaea L. with biotechnological applications.Scientific reports · 2025Article
- Multiscale interface engineering enables strong and water resistant wood bonding.Nature communications · 2025Article
- Structural and mechanical properties of humidity-responsive Geraniaceae awns.Scientific reports · 2025Article
- Citrus Medica-derived Fluorescent Carbon Dots for the Imaging of Vigna Radiate Root Cells.Journal of fluorescence · 2025Article
- Advanced imaging-enabled understanding of cell wall remodeling mechanisms mediating plant drought stress tolerance.Frontiers in plant science · 2025Review
- Modification of xylan in secondary walls alters cell wall biosynthesis and wood formation programs and improves saccharification.Plant biotechnology journal · 2025Article
- Protocol to analyse the structural composition by fluorescence microscopy and different conventional and fluorescence staining methods.MethodsX · 2024Article
- Multimodal imaging analysis in silver fir reveals coordination in cellulose and lignin deposition.Plant physiology · 2024Article
- Two-photon excitation fluorescence microspectroscopy protocols for examining fluorophores in fossil plants.Communications biology · 2024Article
- Stress phenotyping analysis leveraging autofluorescence image sequences with machine learning.Frontiers in plant science · 2024Article
- Moonlight-like proteins are actually cell wall components in Pseudocercospora fijiensis.World journal of microbiology & biotechnology · 2023Article
- The Novel Effector Ue943 Is Essential for Host Plant Colonization byJournal of fungi (Basel, Switzerland) · 2023Article
- Cellulose in Secondary Xylem of Cactaceae: Crystalline Composition and Anatomical Distribution.Polymers · 2022Article
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Investigating plant structure is fundamental in botanical science and provides crucial knowledge for the theories of plant evolution, ecophysiology and for the biotechnological practices. Modern plant anatomy often targets the formation, localization and characterization of cellulosic, lignified or suberized cell walls. While classical methods developed in the 1960s are still popular, recent innovations in tissue preparation, fluorescence staining and microscopy equipment offer advantages to the traditional practices for investigation of the complex lignocellulosic walls. Our goal is to enhance the productivity and quality of microscopy work by focusing on quick and cost-effective preparation of thick sections or plant specimen surfaces and efficient use of direct fluorescent stains. We discuss popular histochemical microscopy techniques for visualization of cell walls, such as autofluorescence or staining with calcofluor, Congo red (CR), fluorol yellow (FY) and safranin, and provide detailed descriptions of our own approaches and protocols. Autofluorescence of lignin in combination with CR and FY staining can clearly differentiate between lignified, suberized and unlignified cell walls in root and stem tissues. Glycerol can serve as an effective clearing medium as well as the carrier of FY for staining of suberin and lipids allowing for observation of thick histological preparations. Three-dimensional (3D) imaging of all cell types together with chemical information by wide-field fluorescence or confocal laser scanning microscopy (CLSM) was achieved.
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