ArticleFrontiers in microbiology2020
Clinical Validation of Two Recombinase-Based Isothermal Amplification Assays (RPA/RAA) for the Rapid Detection of African Swine Fever Virus.
Article in Frontiers in microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 89 papers.
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Who cites it
89 citing papers in PubMed, 152 citations in OpenAlex.
- CRISPR/Cas14a combined with RPA for visual detection of Marek's disease virus.Microbiology spectrum · 2026Article
- Advances in CRISPR-Cas12a/13a-Based Nucleic Acid Detection for Porcine Viral Diseases: A Comprehensive Review.Veterinary sciences · 2026Review
- Systematic review and meta-analysis on the diagnostic accuracy of various detection methods for porcine reproductive and respiratory syndrome virus.Porcine health management · 2026Article
- Establishment of a rapid and highly sensitive direct-RAA-RDB detection platform: application in non-deletion α-thalassemia.Frontiers in molecular biosciences · 2026Article
- The Complexities of African Swine Fever Diagnosis and Control in the Developing World: A Narrative Review Focused on Ghana.Animals : an open access journal from MDPI · 2025Review
- Establishment of an RAA-CRISPR/Cas12a assay based onMicrobiology spectrum · 2025Article
- Establishment of a Rapid and Efficient Method for the Detection of Avian Reovirus Based on RT-RAA-CRISPR/Cas12a Technology.Animals : an open access journal from MDPI · 2025Article
- Development of a novel, rapid, and accurate recombinase polymerase amplification assay for the identification of the glassy-winged sharpshooter,3 Biotech · 2025Article
- Development of Reverse Transcriptase Recombinase Polymerase Amplification Combined with Lateral Flow Dipstick for Rapid Detection of Tilapia Lake Virus (TiLV): Pilot Study.Veterinary sciences · 2025Article
- Multiplex real-time reverse transcription recombinase-aided amplification assay for the detection of SARS-CoV-2, influenza A virus, and respiratory syncytial virus.Microbiology spectrum · 2025Article
- CRISPR/Cas-Based Prenatal Screening for Aneuploidy: Challenges and Opportunities for Early Diagnosis.Medicina (Kaunas, Lithuania) · 2025Review
- Development of Real-Time and Lateral Flow Dipstick Recombinase Polymerase Amplification Assays for the Rapid Field Diagnosis of MGF-505R Gene-Deleted Mutants of African Swine Fever Virus.Veterinary sciences · 2025Article
- Rapid visual detection of hepatitis E virus combining reverse transcription recombinase-aided amplification with lateral flow dipstick and real-time fluorescence.Journal of clinical microbiology · 2025Article
- Integrated Device for Cancer Nucleic Acid Biomarker Detection at Body Temperature.Micromachines · 2025Article
- Development of nucleic acid-based RAA test strip assay for the rapid detection of foot-and-mouth disease virus.Frontiers in veterinary science · 2025Article
- Establishment of reverse transcription recombinase-aided amplification with lateral flow dipstick for the rapid visual detection of Getah virus.Frontiers in cellular and infection microbiology · 2025Article
- A rapid visual detection method for porcine teschovirus through reverse transcription recombinase-aided amplification coupled with lateral flow dipstick.BMC veterinary research · 2024Article
- Pathogenicity and virulence of African swine fever virus.Virulence · 2024Review
- Sex Identification of a Multispecies Carinatae Birds by Chicken EE0.6 Gene Using Real-Time Recombinase-Aid Amplification Assay.Ecology and evolution · 2024Article
- Strategic nucleic acid detection approaches for diagnosing African swine fever (ASF): navigating disease dynamics.Veterinary research · 2024Review
29 more citing papers are in PubMed but not listed here.
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Authors and funding
17 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
African swine fever (ASF), caused by African swine fever virus (ASFV), is a devastating infectious disease of domestic pigs and wild boars, and has tremendous negative socioeconomic impact on the swine industry and food security worldwide. It is characterized as a notifiable disease by World Organisation for Animal Health (OIE). No effective vaccine or treatment against ASF has so far been available. Early detection and rapid diagnosis are of potential significance to control the spread of ASF. Recombinase-based isothermal amplification assay, recombinase polymerase amplification (RPA) developed by TwistDx (Cambridge, United Kingdom) or recombinase-aided amplification (RAA) by Qitian (Wuxi, China), is becoming a molecular tool for the rapid, specific, and cost-effective identification of multiple pathogens. In this study, we aim to investigate if RPA/RAA can be a potential candidate for on-site, rapid and primary detection of ASFV. A panel of 152 clinical samples previously well-characterized by OIE-recommended qPCR was enrolled in this study, including 20 weak positive (Ct value ≥ 30) samples. This panel was consisted of different types, such as EDTA-blood, spleen, lung, lymph node, kidney, tonsil, liver, brain. We evaluated two recombinase-based isothermal amplification assays, RPA or RAA, by targeting the ASFV
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