ArticleFrontiers in microbiology2020
Single-Sampling Strategy vs. Multi-Sampling Strategy for Blood Cultures in Sepsis: A Prospective Non-inferiority Study.
Article in Frontiers in microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Blood culture practices and microbiological capacity for sepsis diagnostics in Europe (2021-2022): a cross-sectional analysis of the European Sepsis Care Survey.The Lancet regional health. Europe · 2026Article
- Detection of relevant pathogens and contaminants in blood cultures after implementation of single-sampling strategy and initial specimen diversion.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2025Article
- [Antibiotic stewardship-an update : Ongoing development of an initiative].Medizinische Klinik, Intensivmedizin und Notfallmedizin · 2025Review
- Comparing contamination rates in paired blood culture sets with a single-time stamp vs multiple-time stamps.Antimicrobial stewardship & healthcare epidemiology : ASHE · 2025Article
- Distinct MAIT cell phenotypes associated with sepsis clinical outcome in emergency department patients.Clinical & translational immunology · 2025Article
- Targeted plasma proteomics reveals signatures discriminating COVID-19 from sepsis with pneumonia.Respiratory research · 2023Article
- Plasma calprotectin as an indicator of need of transfer to intensive care in patients with suspected sepsis at the emergency department.BMC emergency medicine · 2023Article
- Diagnostic value of metagenomic next-generation sequencing in sepsis and bloodstream infection.Frontiers in cellular and infection microbiology · 2023Article
- Correlation of clinical sepsis definitions with microbiological characteristics in patients admitted through a sepsis alert system; a prospective cohort study.Annals of clinical microbiology and antimicrobials · 2022Article
- Single-Site Sampling versus Multisite Sampling for Blood Cultures: a Retrospective Clinical Study.Journal of clinical microbiology · 2022Article
- Observational
- Low prevalence of bloodstream infection and high blood culture contamination rates in patients with COVID-19.PloS one · 2020Article
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9 authors.
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Abstract
backgroundOptimal sampling is critical for the performance of blood cultures (BCs). Most guidelines recommend collecting 40 ml of blood, divided between two venipuncture sites, i.e., multi-sampling strategy (MSS). Sampling through a single venipuncture site, i.e., single-sampling strategy (SSS) is easier; however, the diagnostic performance of SSS compared to MSS remains unknown. Thus, we aimed to study if SSS is non-inferior to MSS for detection of pathogenic microorganisms.
methodsA prospective, paired, non-inferiority design was used. Patients with clinically suspected sepsis admitted to an Emergency Department were included. Six BC bottles were simultaneously collected, consisting of four BC bottles from the first arm and two from the other arm. SSS consisted of BC bottles 1, 2, 3, and 4, and MSS consisted of BC bottles 1, 2, 5, and 6. Samples were incubated in a BacT/ALERT BC system.
resultsThe final analysis included 549 episodes. Pathogenic microorganisms were detected in 162 cases (29.5%) with MSS and 160 cases (29.1%) with SSS, yielding an absolute difference of 0.36%, with a 95% confidence interval of -1.33 to 2.06%, which did not exceed the predefined non-inferiority margin of 5%. MSS tended to produce more contaminant growth (7.3% of cases) than SSS (5.3% of cases;
conclusionThe study showed that SSS was non-inferior to MSS in detecting pathogenic microorganisms and supports the use of SSS as a routine method.
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