ArticleFrontiers in microbiology2020
Metagenomic Insights Into the Diversity of Halophilic Microorganisms Indigenous to the Karak Salt Mine, Pakistan.
Article in Frontiers in microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
34 citing papers in PubMed.
- Taxonomic and Functional Comparative Metagenomics of Peruvian Salterns: Insights into Microbial Communities and Aminotransferase Potential.Microorganisms · 2026Article
- Insights into microbial diversity affected by plant cover in athalassohaline ecosystem of el-hamra lake, wadi el-natrun, Egypt.World journal of microbiology & biotechnology · 2026Article
- Airborne Halophilic and Non-Halophilic Microbial Communities in an Underground Salt Mine Affected by Seasonal Environmental Fluctuations.Environmental microbiology reports · 2026Article
- In situ filtration reveals salinity-driven dynamics of prokaryotic and eukaryotic communities in the Yinggehai solar saltern.Extremophiles : life under extreme conditions · 2026Article
- Rock surfaces as reservoirs for airborne halophilic microorganisms in the Bochnia Salt Mine.Frontiers in microbiology · 2026Article
- Isolation of high yield polyhydroxyalkanoate producing bacteria from contaminated soils and biopolymer characterization.Scientific reports · 2025Article
- 'gitana' (phyloGenetic Imaging Tool for Adjusting Nodes and other Arrangements), a tool for plotting phylogenetic trees into ready-to-publish figures.BMC bioinformatics · 2025Article
- Article
- Prokaryotic Diversity and Community Distribution in the Complex Hydrogeological System of the Añana Continental Saltern.Microbial ecology · 2025Article
- Deep underground metagenome-assembled genomes from hydrothermal spring.Microbiology resource announcements · 2024Article
- Establishing African genomics and bioinformatics programs through annual regional workshops.Nature genetics · 2024Review
- Article
- Exploring Pathogen Presence Prediction in Pastured Poultry Farms through Transformer-Based Models and Attention Mechanism Explainability.Microorganisms · 2024Article
- Metagenomic profiling of halites from the Atacama Desert: an extreme environment with natural perchlorate does not promote high diversity of perchlorate reducing microorganisms.Extremophiles : life under extreme conditions · 2024Article
- Bioprospecting of unexplored halophilic actinobacteria against human infectious pathogens.3 Biotech · 2023Review
- Phylogeny and functional diversity of halophilic microbial communities from a thalasso environment.Saudi journal of biological sciences · 2023Article
- Dynamics of Methane-Consuming Biomes from Wieliczka Formation: Environmental and Enrichment Studies.Biology · 2023Article
- Isolation, microscopic and magnetotactic characterization of Magnetospirillum moscoviense MS-24 from Banjosa Lake, Pakistan.Biotechnology letters · 2023Article
- Bioremediation of environments contaminated with mercury. Present and perspectives.World journal of microbiology & biotechnology · 2023Review
- Decoding the microbial universe with metagenomics: a brief insight.Frontiers in genetics · 2023Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hypersaline regions are terrestrial analogs of the Earth's primitive ecosystem and extraterrestrial environment. The salt range in Pakistan is considered among a few of the ancient salt deposits in the subcontinent. Karak salt mine is situated at the Northwest end in Pakistan. Despite the fact that halophiles initiated the formation of terrestrial ecosystems, their products and identities remain hidden. Some preliminary studies limited to culture-dependent isolations have been reported. Characterizing the microbiome that spans over centuries of ecosystem development is crucial, given their role in shaping landscape succession and biogeochemical cycles. Here, we used metagenomics techniques to explore the microbial diversity of the Karak salt mine. We used 16S rRNA Illumina amplicon sequencing to characterize the halophilic communities entrapped in Karak mine. The results were interpreted using Illumina Basespace, QIIME, and Cytoscape. Cultures were isolated at 16-25% salinity. Metagenomics data was consistent with our preliminary culturing data, indicating remarkable species to strain-level diversity of unique halophiles. A total of 107,099 (brine) and 122,679 (salt) reads were obtained. 16S rRNA based sequencing revealed a microbiome with bacteria (66% brine and 72% salt) dominated by Bacteroidetes and Proteobacteria with a strikingly high abundance of Archaea (18% brine and 13% salt). Alpha diversity has higher values in salt than in the brine. The study of the halophiles in the Karak salt mine provides clues for species contributing to the maintenance of biogeochemical cycles of the ecosystem. This is the first report of a metagenomic study of any hypersaline region of Pakistan.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.