ArticlePloS one2020
Excreta biomarkers in response to different gut barrier dysfunction models and probiotic supplementation in broiler chickens.
Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 27 citations in OpenAlex.
- A NEW FOCUS ON FIBER.Poultry science · 2026Review
- Phytogenic feed additive mitigates necrotic enteritis-associated gut damage and performance loss in broilers.Poultry science · 2026Article
- Association ofFrontiers in cellular and infection microbiology · 2025Article
- Interaction Between Peroxisome Proliferator-Activated Receptors and Cannabidiol in the Gut of Chickens Applied to Different Challenge Conditions.International journal of molecular sciences · 2024Article
- Investigation of Potential Gut Health Biomarkers in Broiler Chicks Challenged byPathogens (Basel, Switzerland) · 2024Article
- Article
- Negative consequences of reduced protein diets supplemented with synthetic amino acids for performance, intestinal barrier function, and caecal microbiota composition of broiler chickens.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2023Article
- Effects of Different Feeding Durations on Ileum Length and Weight and Basal Endogenous Loss of Phosphorus in Broiler Chickens Fed a Phosphorus-Free Diet.Animals : an open access journal from MDPI · 2023Article
- Effects of tannic acid on the immunity and intestinal health of broiler chickens with necrotic enteritis infection.Journal of animal science and biotechnology · 2023Article
- Mechanisms underlying the Effects of Heat Stress on Intestinal Integrity, Inflammation, and Microbiota in Chickens.The journal of poultry science · 2023Review
- Identifying the shortfalls of crude protein-reduced, wheat-based broiler diets.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2022Review
- Cannabidiol affects breast meat volatile compounds in chickens subjected to different infection models.Scientific reports · 2022Article
- Centennial Review: Factors affecting the chicken gastrointestinal microbial composition and their association with gut health and productive performance.Poultry science · 2022Review
- Butyric and Citric Acids and Their Salts in Poultry Nutrition: Effects on Gut Health and Intestinal Microbiota.International journal of molecular sciences · 2021Review
- Current experimental models, assessment and dietary modulations of intestinal permeability in broiler chickens.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2021Review
- Novel Models for Chronic Intestinal Inflammation in Chickens: Intestinal Inflammation Pattern and Biomarkers.Frontiers in immunology · 2021Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Increased intestinal permeability (IP) and inflammation are both linked with functionality of the intestinal barrier and in particular enterocytes. Currently, almost all assessment methods of the intestinal barrier function are invasive. The present study aimed to quantify selected proteins as novel biomarkers in excreta of broiler chickens to facilitate non-invasive assessment of gut barrier function using enzyme-linked immunosorbent assays (ELISA). It was further hypothesised that probiotics as feed additives may counteract gut barrier dysfunction. A 3 × 2 factorial arrangement of treatments was used with the main factors being gut barrier dysfunction models (control, rye-based diet, and dexamethasone-DEX) with and without probiotic supplementation (a three-strain Bacillus) using 72 male Ross 308 day-old chickens. Each of the 6 experimental treatments was replicated 12 times. On d 21 of age, fluorescein isothiocyanate dextran (FITC-d) uptake into serum was examined to test IP. Fresh excreta samples were collected on d 20. The biomarkers included alpha-1 antitrypsin (A1AT), intestinal fatty acid binding protein (I-FABP), lipocalin-2 (LCN2), fibronectin (FN), intestinal alkaline phosphatase (IAP), ovotransferrin (OVT) and superoxide dismutase [Cu-Zn] (SOD1). Only DEX increased (P<0.001) FITC-d passage to the blood on d 21 of age, indicating a greater IP. The excreta concentrations of A1AT, I-FABP and SOD1 were unaltered by the experimental treatments. DEX increased (P<0.05) FN concentration in excreta compared with control birds. Conversely, inclusion of rye in the diet reduced (P<0.05) FN but increased (P<0.001) OVT in excreta. Independently, DEX decreased IAP (P<0.05) in excreta compared with control and rye-fed birds. The excreta concentration of LCN2 tended (P = 0.086) to increase in birds injected by DEX. There was no demonstrable effect of probiotic addition on any of the studied parameters. Among the tested biomarkers, FN, IAP, and LCN2 revealed promise as biomarkers of intestinal barrier function quantified by ELISA kits.
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