Evidence map›Paper›PMID 32789704›Full record

ArticleAMB Express2020

Large-scale production of recombinant miraculin protein in transgenic carrot callus suspension cultures using air-lift bioreactors.

Yun-Ji Park, Jong-Eun Han, Hyoshin Lee, Yu-Jin Jung, Hosakatte Niranjana Murthy, So-Young Park

Open access · goldAbstract read
In one paragraph

Article in AMB Express, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.7field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 16 citations in OpenAlex.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 2 countries.

Yun-Ji ParkDepartment of Horticulture, Division of Animal, Horticultural and Food Sciences, Chungbuk National University, Cheongju, 28644, Republic of Korea.
Jong-Eun HanDepartment of Horticulture, Division of Animal, Horticultural and Food Sciences, Chungbuk National University, Cheongju, 28644, Republic of Korea.
Hyoshin LeeDepartment of Forest Genetic Resources, National Institute of Forest Science, 39 Onjeong-ro, Suwon, 16631, Republic of Korea.
Yu-Jin JungDepartment of Horticultural Life Science, Hankyong National University, Anseong, 17579, Republic of Korea.
Hosakatte Niranjana MurthyDepartment of Horticulture, Division of Animal, Horticultural and Food Sciences, Chungbuk National University, Cheongju, 28644, Republic of Korea.
So-Young ParkDepartment of Horticulture, Division of Animal, Horticultural and Food Sciences, Chungbuk National University, Cheongju, 28644, Republic of Korea. soypark7@cbnu.ac.kr.
Chungbuk National University · KRHankyong National University · KRKorea Forest Service · KR

Funding

Next Generation BioGreen 21 Program PJ013689
6 · The paper itself

Abstract

Miraculin, derived from the miracle fruit (Synsepalum dulcificum), is a taste-regulating protein that interacts with human sweet-taste receptors and transforms sourness into sweet taste. Since miracle fruit is cultivated in West Africa, mass production of miraculin is limited by regional and seasonal constraints. Here, we investigated mass production of recombinant miraculin in carrot (Daucus carota L.) callus cultures using an air-lift bioreactor. To increase miraculin expression, the oxidative stress-inducible SWPA2 promoter was used to drive the expression of miraculin gene under various stress treatments. An 8 h treatment of hydrogen peroxide (H

Indexed as

Agrobacterium-mediated transformationAlternative sweetenerCarrot callus culturesMiraculinPlant cell cultureRecombinant protein

Identifiers

PMID32789704
PMCPMC7426344
OpenAlexW3049013386

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.