Evidence map›Paper›PMID 32789689›Full record

ArticleCellular and molecular life sciences : CMLS2021

Reciprocal regulation of Aurora kinase A and ATIP3 in the control of metaphase spindle length.

Anne Nehlig, Cynthia Seiler, Yulia Steblyanko, Florent Dingli, Guillaume Arras, Damarys Loew, Julie Welburn, Claude Prigent, Marin Barisic, Clara Nahmias

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cellular and molecular life sciences : CMLS, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
1.0field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 17 citations in OpenAlex.

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  7. The Angiotensin ATPharmacological reviews · 2022
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 6 institutions in 3 countries.

Anne NehligInserm U981, Department of Molecular Medicine, Gustave Roussy Cancer Center, 114 rue Edouard Vaillant, 94800, Villejuif, France.
Cynthia SeilerInserm U981, Department of Molecular Medicine, Gustave Roussy Cancer Center, 114 rue Edouard Vaillant, 94800, Villejuif, France.
Yulia SteblyankoCell Division Laboratory, Danish Cancer Society Research Center, 2100, Copenhagen, Denmark.
Florent DingliCentre de Recherche, Laboratoire de Spectrométrie de Masse Protéomique, Institut Curie, PSL Research University, 75248, Paris Cedex 05, France.
Guillaume ArrasCentre de Recherche, Laboratoire de Spectrométrie de Masse Protéomique, Institut Curie, PSL Research University, 75248, Paris Cedex 05, France.
Damarys LoewCentre de Recherche, Laboratoire de Spectrométrie de Masse Protéomique, Institut Curie, PSL Research University, 75248, Paris Cedex 05, France.
Julie WelburnWellcome Trust Centre for Cell Biology, School of Biological Sciences, University of Edinburgh, Edinburgh, Scotland, UK.
Claude PrigentInstitut de Génétique et Développement de Rennes (IGDR), Unité CNRS, UMR 6290, Université de Rennes, 35043, Rennes, France.
Marin BarisicCell Division Laboratory, Danish Cancer Society Research Center, 2100, Copenhagen, Denmark.
Clara NahmiasInserm U981, Department of Molecular Medicine, Gustave Roussy Cancer Center, 114 rue Edouard Vaillant, 94800, Villejuif, France. clara.nahmias@inserm.fr.ORCID http://orcid.org/0000-0001-6863-370X
Inserm · FRUniversité Paris Sciences et Lettres · FRCentre National de la Recherche Scientifique · FRDanish Cancer Society · DKUniversity of Copenhagen · DKWellcome Centre for Cell Biology · GB

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Maintaining the integrity of the mitotic spindle in metaphase is essential to ensure normal cell division. We show here that depletion of microtubule-associated protein ATIP3 reduces metaphase spindle length. Mass spectrometry analyses identified the microtubule minus-end depolymerizing kinesin Kif2A as an ATIP3 binding protein. We show that ATIP3 controls metaphase spindle length by interacting with Kif2A and its partner Dda3 in an Aurora kinase A-dependent manner. In the absence of ATIP3, Kif2A and Dda3 accumulate at spindle poles, which is consistent with reduced poleward microtubule flux and shortening of the spindle. ATIP3 silencing also limits Aurora A localization to the poles. Transfection of GFP-Aurora A, but not kinase-dead mutant, rescues the phenotype, indicating that ATIP3 maintains Aurora A activity on the poles to control Kif2A targeting and spindle size. Collectively, these data emphasize the pivotal role of Aurora kinase A and its mutual regulation with ATIP3 in controlling spindle length.

Indexed as

Aurora Kinase AHeLa CellsHumansKinesinsMetaphaseMicrotubule-Associated ProteinsMicrotubulesMitosisPhosphoproteinsSpindle ApparatusTumor Suppressor ProteinsAurora Kinase AKIF2A protein, humanKinesinsMicrotubule-Associated ProteinsMTUS1 protein, humanPhosphoproteinsPSRC1 protein, humanTumor Suppressor ProteinsAurora ADda3Kif2AMitotic kinaseMitotic spindleMTUS1Poleward microtubule flux

Identifiers

PMID32789689
PMCPMC11072152
OpenAlexW3049144430

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.