ArticlePlant cell reports2020
A peak in global DNA methylation is a key step to initiate the somatic embryogenesis of coconut palm (Cocos nucifera L).
Article in Plant cell reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 29 citations in OpenAlex.
- Understanding epigenetic regulation in nonmodel plants: transcriptomic responses to seed demethylation in leaves and roots of the annual herb Erodium cicutarium (Geraniaceae).G3 (Bethesda, Md.) · 2026Article
- Targeting histone acetylation to enhance somatic embryogenesis in Quercus suber L.Tree physiology · 2025Article
- 5-AzaC Facilitates Somatic Embryogenesis and Germination Across Two Embryogenic Lines inPlants (Basel, Switzerland) · 2025Article
- Morphogenetic Factors as a Tool for Enhancing Plant Regeneration Capacity During In Vitro Transformation.International journal of molecular sciences · 2025Review
- Molecular insights of somatic embryogenesis in coconut.Molecular biology reports · 2025Review
- Conserved stress-responsive genes involved in the early development of Euterpe Edulis.Scientific reports · 2025Article
- Small molecules, enormous functions: potential approach for overcoming bottlenecks in embryogenic tissue induction and maintenance in conifers.Horticulture research · 2024Review
- Quantification of DNA Methylation by ELISA in Epigenetic Studies in Plant Tissue Culture: A Theoretical-Practical Guide.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Cellular responses of oil palm genotypes during somatic embryogenesis involve participation of procambial cells, DNA demethylation, and auxin accumulation.Plant cell reports · 2022Article
- Global DNA methylation and chondrogenesis of rat limb buds in a three-dimensional organ culture system.Bosnian journal of basic medical sciences · 2022Article
- Molecular Determinants ofFrontiers in plant science · 2022Review
- Cloning Coconut via Somatic Embryogenesis: A Review of the Current Status and Future Prospects.Plants (Basel, Switzerland) · 2021Review
- Transcriptomic Time-Series Analyses of Gene Expression Profile During Zygotic Embryo Development inFrontiers in genetics · 2021Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
key messageDNA methylation, morphogenesis and gene expression during the somatic embryogenesis of Coconut are affected by 5-Azacytidine pretreatments, indicating that DNA methylation is an important factor throughout this process. Somatic embryogenesis (SE) is a process that can aid in the production of elite Cocos nucifera palms. It has been well established that epigenetic mechanisms are regulators of cell differentiation programs; however, their role in the coconut somatic embryogenesis has not yet been addressed. To this end, the morphogenetic changes, the global DNA methylation and the expression profiles of the SE-related genes and DNA methyltransferases genes were evaluated during the SE process, with and without the presence of 5-Azacytidine (AzaC). The results show that three days of pretreatments with 15 µM and 20 µM of AzaC significantly increased early somatic embryo formation (four- and tenfold, respectively). A clear peak of the global percentage of DNA methylation (approximately 13%) was determined at the beginning of the culture, followed by a re-establishing stage and a steady increase thereafter; in all cases, the levels of DNA methylation were lower after the pretreatments with AzaC. Additionally, the expression profiles of the SERK, WUS, BBM and LEC genes are modulated during the SE process and the pretreatments with AzaC affect the expression profiles of these genes, even at early stages. Furthermore, increased levels of expression were observed for the genes encoding for DNA methyltransferases (MET, CMT and DRM) at early and late stages of SE, indicating that DNA methylation is an important factor throughout the SE.
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