ArticleScientific reports2020
The P-glycoprotein repertoire of the equine parasitic nematode Parascaris univalens.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 36 citations in OpenAlex.
- Critical role of P-Glycoprotein-9 in ivermectin tolerance in nematodes.PLoS pathogens · 2026Article
- P-glycoprotein-9-mediated multidrug tolerance in Caenorhabditis elegans.Parasites & vectors · 2025Article
- Article
- Article
- Strain-level variations of Dirofilaria immitis microfilariae in two biochemical assays.PloS one · 2024Article
- Getting around the roundworms: Identifying knowledge gaps and research priorities for the ascarids.Advances in parasitology · 2024Article
- Expression of transporter genes in anthelmintic resistant isolates of Haemonchus contortus.Genetics and molecular biology · 2024Article
- Gene co-expression network analysis reveal core responsive genes in Parascaris univalens tissues following ivermectin exposure.PloS one · 2024Article
- Importance of ABC Transporters in the Survival of Parasitic Nematodes and the Prospect for the Development of Novel Control Strategies.Pathogens (Basel, Switzerland) · 2023Review
- Review
- Repertoire of P-glycoprotein drug transporters in the zoonotic nematode Toxocara canis.Scientific reports · 2023Article
- The equine ascarids: resuscitating historic model organisms for modern purposes.Parasitology research · 2022Review
- Transgenic Expression ofInternational journal of molecular sciences · 2022Article
- Ivermectin-induced gene expression changes in adult Parascaris univalens and Caenorhabditis elegans: a comparative approach to study anthelminthic metabolism and resistance in vitro.Parasites & vectors · 2022Article
- Genomics of the Parasitic NematodeGenes · 2021Review
- Pharyngeal Pumping and Tissue-Specific Transgenic P-Glycoprotein Expression Influence Macrocyclic Lactone Susceptibility inPharmaceuticals (Basel, Switzerland) · 2021Article
Corrections and comments
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Authors and funding
11 authors at 6 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
P-glycoproteins (Pgp) have been proposed as contributors to the widespread macrocyclic lactone (ML) resistance in several nematode species including a major pathogen of foals, Parascaris univalens. Using new and available RNA-seq data, ten different genomic loci encoding Pgps were identified and characterized by transcriptome-guided RT-PCRs and Sanger sequencing. Phylogenetic analysis revealed an ascarid-specific Pgp lineage, Pgp-18, as well as two paralogues of Pgp-11 and Pgp-16. Comparative gene expression analyses in P. univalens and Caenorhabditis elegans show that the intestine is the major site of expression but individual gene expression patterns were not conserved between the two nematodes. In P. univalens, PunPgp-9, PunPgp-11.1 and PunPgp-16.2 consistently exhibited the highest expression level in two independent transcriptome data sets. Using RNA-Seq, no significant upregulation of any Pgp was detected following in vitro incubation of adult P. univalens with ivermectin suggesting that drug-induced upregulation is not the mechanism of Pgp-mediated ML resistance. Expression and functional analyses of PunPgp-2 and PunPgp-9 in Saccharomyces cerevisiae provide evidence for an interaction with ketoconazole and ivermectin, but not thiabendazole. Overall, this study established reliable reference gene models with significantly improved annotation for the P. univalens Pgp repertoire and provides a foundation for a better understanding of Pgp-mediated anthelmintic resistance.
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