ArticleProceedings of the National Academy of Sciences of the United States of America2020
When DNA gets in the way: A cautionary note for DNA contamination in extracellular RNA-seq studies.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed.
- Protocol for total RNA sequencing analysis of extracellular RNA from biofluids.STAR protocols · 2026Article
- Exploiting the role of milk extracellular vesicles: a comprehensive analysis on isolation methods, characterization, surface modifications, and their therapeutic applications.Journal of nanobiotechnology · 2026Review
- In-depth comparison of methods to isolate RNA from common human cell lines.BMC genomics · 2026Article
- Subpar reporting of pre-analytical variables in RNA-focused blood plasma studies.Molecular oncology · 2025Review
- Blood collection tube and RNA purification method recommendations for extracellular RNA transcriptome profiling.Nature communications · 2025Article
- Liquid biopsy: paving a new avenue for cancer research.Cell adhesion & migration · 2024Review
- CleanUpRNAseq: An R/Bioconductor Package for Detecting and Correcting DNA Contamination in RNA-Seq Data.Biotech (Basel (Switzerland)) · 2024Article
- Depletion-assisted multiplexed cell-free RNA sequencing reveals distinct human and microbial signatures in plasma versus extracellular vesicles.Clinical and translational medicine · 2024Article
- Use of adeno-associated viruses for transgenic modulation of microglia structure and function: A review of technical considerations and challenges.Brain, behavior, and immunity · 2024Review
- Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches.Journal of extracellular vesicles · 2024Article
- Reverse transcription-quantitative PCR (RT-qPCR) without the need for prior removal of DNA.Scientific reports · 2023Article
- Current challenges and best practices for cell-free long RNA biomarker discovery.Biomarker research · 2022Review
- Genes expressed at low levels raise false discovery rates in RNA samples contaminated with genomic DNA.BMC genomics · 2022Article
- Inferring gene expression from cell-free DNA fragmentation profiles.Nature biotechnology · 2022Article
- Dissecting the transcriptome in cardiovascular disease.Cardiovascular research · 2022Review
- Messenger RNA capture sequencing of extracellular RNA from human biofluids using a comprehensive set of spike-in controls.STAR protocols · 2021Article
- When DNA gets in the way: A cautionary note for DNA contamination in extracellular RNA-seq studies.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Reply to Verwilt et al.: Experimental evidence against DNA contamination in SILVER-seq.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Investigating Pregnancy and Its Complications Using Circulating Cell-Free RNA in Women's Blood During Gestation.Frontiers in pediatrics · 2020Review
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.