ArticleParasites & vectors2020
Natural Wolbachia infection in field-collected Anopheles and other mosquito species from Malaysia.
Article in Parasites & vectors, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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30 citing papers in PubMed, 55 citations in OpenAlex.
- Natural Infection by Toscana Virus and Other Phleboviruses in Wild Sand Flies From Spain: Prevalence, Genetic Characterization and Tripartite Interactions With Leishmania infantum and Wolbachia.Zoonoses and public health · 2026Article
- Identifying Transgenic Approaches to Mitigate Vector Transmission of Malaria by Anopheles gambiae.Cureus · 2026Review
- The symbiotic Wolbachia in Anopheles and its role in reducing the transmission of Plasmodium: updates and prospects.Archives of microbiology · 2026Review
- Advancing Malaria Vector Control: Insights Into Mosquito Immunity and Genetic Strategies.TheScientificWorldJournal · 2026Review
- Molecular Identification and Phylogeny of NaturalJournal of tropical medicine · 2026Article
- Extracellular microbes are required for mosquito development even in the presence of Wolbachia.PLoS neglected tropical diseases · 2025Article
- The Effect of Larval Exposure to Plastic Pollution on the Gut Microbiota of the Major Malaria Vector Anopheles arabiensis Patton (Diptera: Culicidae).Environmental microbiology reports · 2025Article
- Unstable laboratory Wolbachia strain w-Anga is negatively correlated with Plasmodium falciparum in wild malaria vectors.Scientific reports · 2025Article
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- Gene drives: an alternative approach to malaria control?Gene therapy · 2025Review
- Symbiotic Bacteria: Wolbachia, Midgut Microbiota in Mosquitoes and Their Importance for Vector Prevention Strategies.Microbial ecology · 2024Review
- Mosquito Gut Microbiota: A Review.Pathogens (Basel, Switzerland) · 2024Review
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- RevisitingEcology and evolution · 2024Article
- Diversity of Wolbachia infections in Sri Lankan mosquitoes with a new record of Wolbachia Supergroup B infecting Aedes aegypti vector populations.Scientific reports · 2024Article
- Wolbachia infection-responsive immune genes suppress Plasmodium falciparum infection in Anopheles stephensi.PLoS pathogens · 2024Article
- Article
- Updates on traditional methods for combating malaria and emergingFrontiers in cellular and infection microbiology · 2024Review
- Characterizing the Wolbachia infection in field-collected Culicidae mosquitoes from Hainan Province, China.Parasites & vectors · 2023Article
- Genome-wide detection ofFrontiers in cellular and infection microbiology · 2023Article
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10 authors at 2 institutions in 1 country.
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Abstract
backgroundThe endosymbiont bacterium Wolbachia is maternally inherited and naturally infects some filarial nematodes and a diverse range of arthropods, including mosquito vectors responsible for disease transmission in humans. Previously, it has been found infecting most mosquito species but absent in Anopheles and Aedes aegypti. However, recently these two mosquito species were found to be naturally infected with Wolbachia. We report here the extent of Wolbachia infections in field-collected mosquitoes from Malaysia based on PCR amplification of the Wolbachia wsp and 16S rRNA genes.
methodsThe prevalence of Wolbachia in Culicinae mosquitoes was assessed via PCR with wsp primers. For some of the mosquitoes, in which the wsp primers failed to amplify a product, Wolbachia screening was performed using nested PCR targeting the 16S rRNA gene. Wolbachia sequences were aligned using Geneious 9.1.6 software, analyzed with BLAST, and the most similar sequences were downloaded. Phylogenetic analyses were carried out with MEGA 7.0 software. Graphs were drawn with GraphPad Prism 8.0 software.
resultsA total of 217 adult mosquitoes representing 26 mosquito species were screened. Of these, infections with Wolbachia were detected in 4 and 15 mosquito species using wsp and 16S rRNA primers, respectively. To our knowledge, this is the first time Wolbachia was detected using 16S rRNA gene amplification, in some Anopheles species (some infected with Plasmodium), Culex sinensis, Culex vishnui, Culex pseudovishnui, Mansonia bonneae and Mansonia annulifera. Phylogenetic analysis based on wsp revealed Wolbachia from most of the mosquitoes belonged to Wolbachia Supergroup B. Based on 16S rRNA phylogenetic analysis, the Wolbachia strain from Anopheles mosquitoes were more closely related to Wolbachia infecting Anopheles from Africa than from Myanmar.
conclusionsWolbachia was found infecting Anopheles and other important disease vectors such as Mansonia. Since Wolbachia can affect its host by reducing the life span and provide resistance to pathogen infection, several studies have suggested it as a potential innovative tool for vector/vector-borne disease control. Therefore, it is important to carry out further studies on natural Wolbachia infection in vector mosquitoes' populations as well as their long-term effects in new hosts and pathogen suppression.
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