ArticleReproductive biology and endocrinology : RB&E2020
Effect of transient scrotal hyperthermia on human sperm: an iTRAQ-based proteomic analysis.
Article in Reproductive biology and endocrinology : RB&E, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 33 citations in OpenAlex.
- Does Heat-Induced Activation of Mast Cells Contribute to Elevated Temperature-Induced Male Infertility?Pathophysiology : the official journal of the International Society for Pathophysiology · 2026Article
- Comparative Study on the Sperm Proteomes of Horses and Donkeys.Animals : an open access journal from MDPI · 2024Article
- The role of HnrnpF/H as a driver of oligoteratozoospermia.iScience · 2024Article
- Decrease in Sperm Parameters in the 21st Century: Obesity, Lifestyle, or Environmental Factors? An Updated Narrative Review.Journal of personalized medicine · 2024Review
- Differential Proteomic Analysis of Human Sperm: A Systematic Review to Identify Candidate Targets to Monitor Sperm Quality.The world journal of men's health · 2024Article
- The role of the intrinsic pathway of apoptosis in human ejaculated sperm damage under a state of scrotal heat stress.Journal of assisted reproduction and genetics · 2024Article
- Retinoic acid loaded with chitosan nanoparticles improves spermatogenesis in scrotal hyperthermia in mice.Clinical and experimental reproductive medicine · 2023Article
- New Insights on Sperm Function in Male Infertility of Unknown Origin: A Multimodal Approach.Biomolecules · 2023Article
- iTRAQ-based comparative proteomics reveal an enhancing role of PRDX6 in the freezability of Mediterranean buffalo sperm.BMC genomics · 2023Article
- Effects of Heat Stress on Motion Characteristics and Metabolomic Profiles of Boar Spermatozoa.Genes · 2022Article
- The Impact of High Ambient Temperature on Human Sperm Parameters: A Meta-Analysis.Iranian journal of public health · 2022Review
- Global warming and testis function: A challenging crosstalk in an equally challenging environmental scenario.Frontiers in cell and developmental biology · 2022Review
- Occupational Heat Exposure and Fertility Stress as Independent Factors of Low Semen Quality: A Multidimensional Analysis.American journal of men's healthArticle
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
backgroundThrough this prospective study, we aimed to explore the change of molecular modification after the transient scrotal hyperthermia on human sperm.
methodsTen healthy subjects selected with strict screening criteria underwent testicular warming in a 43 °C water bath for 30 min a day for 10 consecutive days. Semen samples were collected 2 weeks before the first heat treatment and 6 weeks after the first heat treatment. Proteins from the samples were labeled with isobaric tags for relative and absolute quantitation and analyzed by two-dimensional liquid chromatography-tandem mass spectrometry.
resultsIn contrast to the control, of the 3446 proteins identified, 61 proteins were deregulated: 28 were up-regulated and 33 were down-regulated. Approximately 95% of the differentially expressed proteins were found to participate in spermatogenesis, fertilization, or other aspects of reproduction. In particular, the expression of sperm motility and energy metabolism-related proteins AKAP4, SPESP1, ODF1, ODF2, GAPDHS, and ACTRT2, validated by western blotting of the proteins obtained from human and mouse samples, tended to be reduced under scrotal hyperthermia.
conclusionsThe results indicated that the proteins AKAP4, ODF1, ODF2, GAPDHS, SPESP1, and ACTRT2, play an important role in the heat-induced reversible reduction in sperm concentration and motility and have the potential to be the biomarkers and clinical targets for scrotal heat treatment induced male infertility.
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