Evidence map›Paper›PMID 32787800›Full record

ArticleBMC cancer2020

Increasing of malignancy of breast cancer cells after cryopreservation: molecular detection and activation of angiogenesis after CAM-xenotransplantation.

Xinxin Du, Plamen Todorov, Evgenia Isachenko, Gohar Rahimi, Peter Mallmann, Yuanguang Meng, Vladimir Isachenko

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC cancer, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
1.0field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 8 citations in OpenAlex.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 3 countries.

Xinxin DuResearch Group for Reproductive Medicine, IVF-Laboratory and Department of Gynecology, University of Cologne, Kerpener str. 34, 50931, Cologne, NRW, Germany.
Plamen TodorovInstitute of Biology and Immunology of Reproduction, Bulgarian Academy of Sciences, Sofia, Bulgaria.
Evgenia IsachenkoResearch Group for Reproductive Medicine, IVF-Laboratory and Department of Gynecology, University of Cologne, Kerpener str. 34, 50931, Cologne, NRW, Germany.
Gohar RahimiResearch Group for Reproductive Medicine, IVF-Laboratory and Department of Gynecology, University of Cologne, Kerpener str. 34, 50931, Cologne, NRW, Germany.
Peter MallmannResearch Group for Reproductive Medicine, IVF-Laboratory and Department of Gynecology, University of Cologne, Kerpener str. 34, 50931, Cologne, NRW, Germany.
Yuanguang MengDepartment of Obstetrics and Gynecology, PLA General Hospital, Beijing, China.
Vladimir IsachenkoResearch Group for Reproductive Medicine, IVF-Laboratory and Department of Gynecology, University of Cologne, Kerpener str. 34, 50931, Cologne, NRW, Germany. vladimir.isachenko@gmail.com.ORCID http://orcid.org/0000-0002-3674-543X
University of Cologne · DEBulgarian Academy of Sciences · BGChinese PLA General Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundOvarian tissue cryopreservation has a wide range of cancerous indications. Avoiding relapse becomes a specific concern that clinicians frequently encounter. The data about the comparative viability of cancer cells after cryopreservation are limited. This study aimed to evaluate the effect of cryopreservation on breast cancer cells.

methodsWe used in-vitro cultured ZR-75-1 and MDA-MB-231 cell lines. Cell samples of each lineage were distributed into the non-intervened and cryopreserved groups. The cryopreservation procedures comprised programmed slow freezing followed by thawing at 100 °C, 60 s. Biological phenotypes and the related protein markers were compared between the two groups. The EVOS FL Auto 2 Cell Image System was used to monitor cell morphology. Cell proliferation, motility, and penetration were characterized by CCK-8, wound-healing, and transmembrane assay, respectively. The expression of Ki-67, P53, GATA3, E-cadherin, Vimentin, and F-Actin was captured by immunofluorescent staining and western blotting as the proxy measurements of the related properties. The chorioallantoic membrane (CAM) xenotransplantation was conducted to explore angiogenesis induced by cancer cells.

resultsAfter 5 days in vitro culture, the cell concentration of cryopreserved and non-intervened groups was 15.7 × 10

conclusionsCryopreservation promotes breast cancer cells in terms of epithelial-mesenchymal transition and angiogenesis induction, thus increasing metastasis risk.

Indexed as

ActinsCryopreservationEpithelial-Mesenchymal TransitionBreast NeoplasmsCadherinsCell AdhesionCell Line, TumorCell MovementCell ProliferationCell ShapeFemaleGATA3 Transcription FactorHumansKi-67 AntigenNeoplasm InvasivenessNeoplasm ProteinsActinsCadherinsGATA3 protein, humanGATA3 Transcription FactorKi-67 AntigenMKI67 protein, humanNeoplasm ProteinsTumor Suppressor Protein p53VimentinAngiogenesisBreast cancerChorioallantoic membraneCryopreservationEpithelial-mesenchymal transitionMetastasis

Identifiers

PMID32787800
PMCPMC7425039
OpenAlexW3048749744

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.