ArticleJournal of the American Chemical Society2020
Multicomponent Bioluminescence Imaging with a π-Extended Luciferin.
Article in Journal of the American Chemical Society, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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Who cites it
17 citing papers in PubMed, 51 citations in OpenAlex.
- Brighter NIR Bioluminescence System for Mammalian Cell Bioimaging Based on Engineered Railroadworm Luciferase and 6'-Aminoluciferin Analogues.Chemical & biomedical imaging · 2026Article
- In vivo ultrasound-induced luminescence imaging via trianthracene derivatives nanomaterials.Nature protocols · 2026Review
- Fluorescent Protein Chromophore-Based Luciferins for Bioluminescence Imaging.Chemical & biomedical imaging · 2025Article
- Bioluminescent Systems for Theranostic Applications.International journal of molecular sciences · 2024Review
- Bright Red Bioluminescence from Semisynthetic NanoLuc (sNLuc).ACS chemical biology · 2024Article
- Biochemical Analysis Leads to Improved Orthogonal Bioluminescent Tools.Chembiochem : a European journal of chemical biology · 2023Article
- Red-Shifted Coumarin Luciferins for Improved Bioluminescence Imaging.Journal of the American Chemical Society · 2023Article
- Multiplexed bioluminescence imaging with a substrate unmixing platform.Cell chemical biology · 2022Article
- Computational Investigation of Substituent Effects on the Fluorescence Wavelengths of Oxyluciferin Analogs.Journal of photochemistry and photobiology. A, Chemistry · 2022Article
- Brightening up Biology: Advances in Luciferase Systems forACS chemical biology · 2021Review
- Coumarin luciferins and mutant luciferases for robust multi-component bioluminescence imaging.Chemical science · 2021Article
- Multicomponent Bioluminescence Imaging with Naphthylamino Luciferins.Chembiochem : a European journal of chemical biology · 2021Article
- Rapid Multicomponent Bioluminescence ImagingACS chemical biology · 2021Article
- Orthogonal Bioluminescent Probes from Disubstituted Luciferins.Biochemistry · 2021Article
- How to Select Firefly Luciferin Analogues for In Vivo Imaging.International journal of molecular sciences · 2021Review
- Color-tunable bioluminescence imaging portfolio for cell imaging.Scientific reports · 2021Article
- Advanced Bioluminescence System for In Vivo Imaging with Brighter and Red-Shifted Light Emission.International journal of molecular sciences · 2020Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Bioluminescence imaging with luciferase-luciferin pairs is commonly used for monitoring biological processes in cells and whole organisms. Traditional bioluminescent probes are limited in scope, though, as they cannot be easily distinguished in biological environments, precluding efforts to visualize multicellular processes. Additionally, many luciferase-luciferin pairs emit light that is poorly tissue penetrant, hindering efforts to visualize targets in deep tissues. To address these issues, we synthesized a set of π-extended luciferins that were predicted to be red-shifted luminophores. The scaffolds were designed to be rotationally labile such that they produced light only when paired with luciferases capable of enforcing planarity. A luciferin comprising an intramolecular "lock" was identified as a viable light-emitting probe. Native luciferases were unable to efficiently process the analog, but a complementary luciferase was identified via Rosetta-guided enzyme design. The unique enzyme-substrate pair is red-shifted compared to well-known bioluminescent tools. The probe set is also orthogonal to other luciferase-luciferin probes and can be used for multicomponent imaging. Four substrate-resolved luciferases were imaged in a single session. Collectively, this work provides the first example of Rosetta-guided design in engineering bioluminescent tools and expands the scope of orthogonal imaging probes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.