ArticleBiochemistry2020
Enzyme Stabilization by Virus-Like Particles.
Article in Biochemistry, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed.
- Pleomorphism in Wild-Type and Engineered PP7 Virus-Like Particles.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Article
- Exploring Use of a Protein Cage System for Producing Bioactive Peptides in Escherichia coli.Microbial biotechnology · 2025Article
- Injectable Hydrogels for Programmable Nanoparticle Release.Advanced functional materials · 2025Article
- Encapsulation of Candida antarctica lipase B in metal-organic framework under ultrasound and using it to one-pot synthesis of 1,3,4,5-tetrasubstituted pyrazoles.Bioprocess and biosystems engineering · 2024Article
- Determining the Serum Stability of Human Adenosine Deaminase 1 Enzyme.Journal of visualized experiments : JoVE · 2024Article
- Article
- Virus-like particle encapsulation of functional proteins: advances and applications.Theranostics · 2024Review
- A Novel Formulation of Asparaginase Encapsulated into Virus-like Particles of Brome Mosaic Virus: In Vitro and In Vivo Evidence.Pharmaceutics · 2023Article
- Sequential Two-Photon Delayed Fluorescence Anisotropy for Macromolecular Size Determination.The journal of physical chemistry. B · 2023Article
- Targeted Enzymatic VLP-Nanoreactors with β-Glucocerebrosidase Activity as Potential Enzyme Replacement Therapy for Gaucher's Disease.ChemMedChem · 2022Article
- Bioconjugation Strategies for Tobacco Mild Green Mosaic Virus.Chembiochem : a European journal of chemical biology · 2022Article
- Transport of Molecular Cargo by Interaction with Virus-Like Particle RNA.Angewandte Chemie (International ed. in English) · 2022Article
- Artificial Self-assembling Nanocompartment for Organizing Metabolic Pathways in Yeast.ACS synthetic biology · 2021Article
- Biophysical characterization of the inactivation of E. coli transketolase by aqueous co-solvents.Scientific reports · 2021Article
- Polyelectrolyte Encapsulation and Confinement within Protein Cage-Inspired Nanocompartments.Pharmaceutics · 2021Review
- Artificial Organelles: Towards Adding or Restoring Intracellular Activity.Chembiochem : a European journal of chemical biology · 2021Review
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
The properties of enzymes packaged within the coat protein shell of virus-like particles (VLPs) were studied to provide a comprehensive assessment of such factors. Such entrainment did not seem to perturb enzyme function, but it did significantly enhance enzyme stability against several denaturing stimuli such as heat, organic solvents, and chaotropic agents. This improvement in performance was found to be general and independent of the number of independent subunits required and of the number of catalytically active enzymes packaged. Packaged enzymes were found by measurements of intrinsic tryptophan fluorescence to retain some of their native folded structure even longer than their catalytic activity, suggesting that protein folding is a significant component of the observed catalytic benefits. While we are unable to distinguish between kinetic and thermodynamic effects - including inhibition of enzyme unfolding, acceleration of refolding, and biasing of folding equilibria - VLP packaging appears to represent a useful general strategy for the stabilization of enzymes that operate on diffusible substrates and products.
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Registered trials
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