Evidence map›Paper›PMID 32786000›Full record

ArticleBiopolymers2021

RNA-based fluorescent biosensors for live cell detection of bacterial sRNA.

Rebekah Z Kitto, Kylee E Christiansen, Ming C Hammond

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biopolymers, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 9 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Rebekah Z KittoDepartment of Chemistry, University of California, Berkeley, California, USA.
Kylee E ChristiansenDepartment of Chemistry and Henry Eyring Center for Cell and Genome Sciences, University of Utah, Salt Lake City, Utah, USA.
Ming C HammondDepartment of Chemistry, University of California, Berkeley, California, USA.ORCID https://orcid.org/0000-0003-2666-4764
University of Utah · US

Funding

Enabling High-Throughput Analysis and Single-Cell Imaging of Bacterial SignalsR01GM124589 · NIGMS · UTAH STATE HIGHER EDUCATION SYSTEM--UNIVERSITY OF UTAH · PI HAMMOND, MING CHEN · 2017 to 2025
$2.8M
Program for Interdisciplinary Training in CHemical BiologyT32GM122740 · NIGMS · UNIVERSITY OF UTAH · PI KAY, MICHAEL S · 2018 to 2022
$932k
Division of Molecular and Cellular Biosciences 1815508NIGMS NIH HHS R01 GM124589NIGMS NIH HHS R01GM124589NIGMS NIH HHS T32 GM122740NIGMS NIH HHS T32GM122740
6 · The paper itself

Abstract

Bacteria contain a diverse set of RNAs to provide tight regulation of gene expression in response to environmental stimuli. Bacterial small RNAs (sRNAs) work in conjunction with protein cofactors to bind complementary mRNA sequences in the cell, leading to up- or downregulation of protein synthesis. In vivo imaging of sRNAs can aid in understanding their spatiotemporal dynamics in real time, which inspires new ways to manipulate these systems for a variety of applications including synthetic biology and therapeutics. Current methods for sRNA imaging are quite limited in vivo and do not provide real-time information about fluctuations in sRNA levels. Herein, we describe our efforts toward the development of an RNA-based fluorescent biosensor for bacterial sRNA both in vitro and in vivo. We validated these sensors for three different bacterial sRNAs in Escherichia coli and demonstrated that the designs provide a bright, sequence-specific signal output in response to exogenous and endogenous RNA targets.

Indexed as

Aptamers, NucleotideBinding SitesBiosensing TechniquesEscherichia coliEscherichia coli ProteinsFlow CytometryFluorescent DyesHost Factor 1 ProteinNucleic Acid HybridizationRNA, BacterialSpectrometry, FluorescenceAptamers, NucleotideEscherichia coli ProteinsFluorescent DyesHfq protein, E coliHost Factor 1 ProteinRNA, Bacterialbiosensorflow cytometrysRNA

Identifiers

PMID32786000
PMCPMC7856060
OpenAlexW3048619568

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.