ArticleClinical rheumatology2021
Identifying the hub gene and immune infiltration of osteoarthritis by bioinformatical methods.
Article in Clinical rheumatology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 39 citations in OpenAlex.
- PRKACB Attenuates Chondrocyte Loss and Inflammation in Osteoarthritis.Immunity, inflammation and disease · 2026Article
- Biologic and regenerative strategies for osteoarthritis: advances in MSC, exosome, and PRP based therapies.Frontiers in pharmacology · 2026Review
- Multi-omics machine learning identifies diagnostic gene signatures and functionally supports PRKACB involvement in macrophage inflammatory responses in sepsis.Frontiers in immunology · 2025Article
- Identification of Key lncRNAs, circRNAs, and mRNAs in Osteoarthritis via Bioinformatics Analysis.Molecular biotechnology · 2024Article
- Identification of hub genes and therapeutic siRNAs to develop novel adjunctive therapy for Duchenne muscular dystrophy.BMC musculoskeletal disorders · 2024Article
- Article
- Identifying Key Genes and Functionally Enriched Pathways in Osteoporotic Patients by Weighted Gene Co-Expression Network Analysis.Biochemical genetics · 2024Article
- Identification of immune microenvironment subtypes and clinical risk biomarkers for osteoarthritis based on a machine learning model.Frontiers in molecular biosciences · 2024Article
- Article
- Identification of hub genes and candidate herbal treatment in obesity through integrated bioinformatic analysis and reverse network pharmacology.Scientific reports · 2022Article
- A Novel Hypoxia Related Marker in Blood Link to Aid Diagnosis and Therapy in Osteoarthritis.Genes · 2022Article
- Constructing a competing endogenous RNA network for osteoarthritis.Annals of translational medicine · 2022Article
- Unraveling the Signaling Secretome of Platelet-Rich Plasma: Towards a Better Understanding of Its Therapeutic Potential in Knee Osteoarthritis.Journal of clinical medicine · 2022Article
- Identification of Biomarkers Associated with Diagnosis of Osteoarthritis Patients Based on Bioinformatics and Machine Learning.Journal of immunology research · 2022Article
- Identification of key genes involved in the recurrence of glioblastoma multiforme using weighted gene co-expression network analysis and differential expression analysis.Bioengineered · 2021Article
- Identifying hub genes and immune infiltration of osteoarthritis using comprehensive bioinformatics analysis.Journal of orthopaedic surgery and research · 2021Article
- Recent Advances in Pharmacological Intervention of Osteoarthritis: A Biological Aspect.Frontiers in pharmacology · 2021Review
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Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundNowadays, there are more and more people who have been diagnosed osteoarthritis (OA). However, due to the complex changes of OA, the treatment outcome is not very well. In order to improve this situation, I decided to aggregate a series of data and use complex bioinformatical methods to analyze them, hoping to explore new therapeutic targets.
methodsAfter downloading and processing the data from Gene Expression Omnibus (GEO) database, I analyzed the relationship between genes and OA formation by the weighted correlation network analysis (WGCNA)and selected the turquoise module which owned the closest relationship with clinical traits. Then, via online database and CIBERSORT algorithm method, I analyzed the function of this key module and the situation of immune infiltration in OA tissues.
resultsWith the help of WGCNA and functional enrichment analysis, I found out that most of genes in the turquoise module took part in the inflammation development, immune responses, and cell proliferation, especially the hub gene PRKACB. At the same time, my results of immune infiltration and expression level analysis also showed that PRKACB has a close relationship with immune cells, especially M2 macrophage.
conclusionIn a word, my results suggested that PRKACB played an essential role in osteoarthritis development. Key Points • Used the "sva" R package to combine the data of 59 samples from four studies to do the bioinformatical analysis. • Identifying the hub gene PRKACB as potential marker for OA and using validation sets to confirm it. • Detecting the situation of immune infiltration in synovium by CIBERSORT algorithm method.
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