ArticleMolecular biology and evolution2021
Spatial Transcriptomics of Nematodes Identifies Sperm Cells as a Source of Genomic Novelty and Rapid Evolution.
Article in Molecular biology and evolution, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers, 1 of them a synthesis that pooled it.
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Who cites it
34 citing papers in PubMed, 1 synthesis or guideline pooled it, 52 citations in OpenAlex.
- An update on the role and potential mechanisms of clock genes regulating spermatogenesis: A systematic review of human and animal experimental studies.Reviews in endocrine & metabolic disorders · 2023Pooled it
- Hookworm genes encoding intestinal excreted-secreted proteins are transcriptionally upregulated in response to the host's immune system.PLoS neglected tropical diseases · 2026Article
- Orphan and de novo Genes in Fungi and Animals: Identification, Origins and Functions.Genome biology and evolution · 2025Review
- Article
- Evolutionary Analysis of Gene-Expression Localization in the Model Crustacean, Daphnia pulex.Molecular ecology · 2025Article
- Interspecies systems biology links bacterial metabolic pathways to nematode gene expression, chemotaxis behavior, and survival.Genome research · 2025Article
- A NovelBiomolecules · 2025Article
- Evolutionary Analysis of Gene-expression Localization in the Model Crustacean,bioRxiv : the preprint server for biology · 2025Article
- High Nutritional Conditions Influence Feeding Plasticity in Pristionchus pacificus and Render Worms Non-Predatory.Journal of experimental zoology. Part B, Molecular and developmental evolution · 2025Article
- A transcriptomic hourglass in brown algae.Nature · 2024Article
- Cryosectioning and immunofluorescence of C. elegans reveals endogenous polyphosphate in intestinal endo-lysosomal organelles.Cell reports methods · 2024Article
- Comparative Genomics of Sex, Chromosomes, and Sex Chromosomes in Caenorhabditis elegans and Other Nematodes.Methods in molecular biology (Clifton, N.J.) · 2024Review
- Co-option of an Astacin Metalloprotease Is Associated with an Evolutionarily Novel Feeding Morphology in a Predatory Nematode.Molecular biology and evolution · 2023Article
- Miniature spatial transcriptomics for studying parasite-endosymbiont relationships at the micro scale.Nature communications · 2023Article
- A Mediator subunit imparts robustness to a polyphenism decision.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Review
- Thousands of Pristionchus pacificus orphan genes were integrated into developmental networks that respond to diverse environmental microbiota.PLoS genetics · 2023Article
- Evolution and implications of de novo genes in humans.Nature ecology & evolution · 2023Review
- The nematode Oscheius tipulae as a genetic model for programmed DNA elimination.Current biology : CB · 2022Article
- RNA-Sequencing of Heterorhabditis nematodes to identify factors involved in symbiosis with Photorhabdus bacteria.BMC genomics · 2022Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Divergence of gene function and expression during development can give rise to phenotypic differences at the level of cells, tissues, organs, and ultimately whole organisms. To gain insights into the evolution of gene expression and novel genes at spatial resolution, we compared the spatially resolved transcriptomes of two distantly related nematodes, Caenorhabditis elegans and Pristionchus pacificus, that diverged 60-90 Ma. The spatial transcriptomes of adult worms show little evidence for strong conservation at the level of single genes. Instead, regional expression is largely driven by recent duplication and emergence of novel genes. Estimation of gene ages across anatomical structures revealed an enrichment of novel genes in sperm-related regions. This provides first evidence in nematodes for the "out of testis" hypothesis that has been previously postulated based on studies in Drosophila and mammals. "Out of testis" genes represent a mix of products of pervasive transcription as well as fast evolving members of ancient gene families. Strikingly, numerous novel genes have known functions during meiosis in Caenorhabditis elegans indicating that even universal processes such as meiosis may be targets of rapid evolution. Our study highlights the importance of novel genes in generating phenotypic diversity and explicitly characterizes gene origination in sperm-related regions. Furthermore, it proposes new functions for previously uncharacterized genes and establishes the spatial transcriptome of Pristionchus pacificus as a catalog for future studies on the evolution of gene expression and function.
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Registered trials
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