ArticlePloS one2020
Creation of novel alleles of fragrance gene OsBADH2 in rice through CRISPR/Cas9 mediated gene editing.
Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 119 citations in OpenAlex.
- CRISPR/Cas-based genome editing for enhancing grain quality traits in rice (Plant signaling & behavior · 2026Review
- Enhanced Rice Yellow Mottle Virus Resistance via CRISPR/Cas9-Targeted Mutagenesis of the Rice eIF(iso)4G Gene.Molecular plant pathology · 2026Article
- Article
- Advances in CRISPR Plant Applications.International journal of molecular sciences · 2026Review
- Harnessing neo-domestication of wild pigmented rice for enhanced nutrition and sustainable agriculture.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Review
- Microbiome Engineering for Sustainable Rice Production: Strategies for Biofertilization, Stress Tolerance, and Climate Resilience.Microorganisms · 2025Review
- Improving the Aroma of Millets by Targeting theCurrent genomics · 2025Review
- Genome editing for sustainable agriculture in Peru: advances, potential applications and regulation.Frontiers in genome editing · 2025Review
- Synthetic biology in plants.Plant biotechnology (Tokyo, Japan) · 2024Article
- Profiling and Improvement of Grain Quality Traits for Consumer Preferable Basmati Rice in the United States.Plants (Basel, Switzerland) · 2024Review
- Flavor profiling and gene expression studies of indigenous aromatic rice variety (Mushk Budiji) grown at different altitudes of Highland Himalayan regions.Scientific reports · 2024Article
- Review
- Genetic modification strategies for enhancing plant resilience to abiotic stresses in the context of climate change.Functional & integrative genomics · 2023Review
- CRISPR/Cas9 gene editing technology: a precise and efficient tool for crop quality improvement.Planta · 2023Review
- CRISPR/Cas9-mediated editing of phytoene desaturase (3 Biotech · 2023Article
- Aroma Components in Horticultural Crops: Chemical Diversity and Usage of Metabolic Engineering for Industrial Applications.Plants (Basel, Switzerland) · 2023Review
- A fast breeding strategy creates fragrance- and anthocyanin-enriched rice lines by marker-free gene-editing and hybridization.Molecular breeding : new strategies in plant improvement · 2023Article
- CRISPR/Cas9-mediated editing ofMolecular breeding : new strategies in plant improvement · 2023Article
- Review
- Creation of Two-Line Fragrant Glutinous Hybrid Rice by Editing theInternational journal of molecular sciences · 2023Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fragrance in rice grains is a key quality trait determining its acceptability and marketability. Intensive research on rice aroma identified mutations in betaine aldehyde dehydrogenase (OsBADH2) leading to production of aroma in rice. Gene editing technologies like CRISPR/Cas9 system has opened new avenues for accelerated improvement of rice grain quality through targeted mutagenesis. In this study, we have employed CRISPR/Cas9 tool to create novel alleles of OsBADH2 leading to introduction of aroma into an elite non-aromatic rice variety ASD16. PCR analysis of putative transformants using primers targeting the flanking regions of sgRNA in the 7th exon of OsBADH2 identified 37.5% potential multi-allelic mutations in T0 generation. Sensory evaluation test in the leaves of T0 lines identified thirteen lines belonging to five independent events producing aroma. Sequence analysis of these aromatic T0 lines identified 22 different types of mutations located within -17 bp to +15bp of sgRNA region. The -1/-2 bp deletion in the line # 8-19 and -8/-5 bp deletion in the line # 2-16 produced strong aroma and the phenotype was stably inherited in the T1 generation. Comparative volatile profiling detected novel aromatic compounds viz., pyrrolidine, pyridine, pyrazine, pyradazine and pyrozole in the grains of T1 progenies of line # 8-19. This study has demonstrated the use of CRISPR/Cas9 in creating novel alleles of OsBADH2 to introduce aroma into any non-aromatic rice varieties.
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