Evidence map›Paper›PMID 32784848›Full record

ArticleMethods and protocols2020

Transfection of hPSC-Cardiomyocytes Using Viafect™ Transfection Reagent.

Sara E Bodbin, Chris Denning, Diogo Mosqueira

Open access · goldAbstract read
In one paragraph

Article in Methods and protocols, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.7field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 12 citations in OpenAlex.

  1. Article
  2. Autonomous multicolor bioluminescence imaging in bacteria, mammalian, and plant hosts.Proceedings of the National Academy of Sciences of the United States of America · 2024
    Article
  3. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Sara E BodbinDivision of Cancer & Stem Cells, Biodiscovery Institute, University of Nottingham, Nottingham NG7 2RD, UK.ORCID 0000-0001-6054-7014
Chris DenningDivision of Cancer & Stem Cells, Biodiscovery Institute, University of Nottingham, Nottingham NG7 2RD, UK.ORCID 0000-0003-0802-8617
Diogo MosqueiraDivision of Cancer & Stem Cells, Biodiscovery Institute, University of Nottingham, Nottingham NG7 2RD, UK.ORCID 0000-0002-2306-3915
University of Nottingham · GB

Funding

British Heart Foundation PG/14/59/31000British Heart Foundation RG/14/1/30588British Heart Foundation SP/15/9/31605British Heart Foundation SP/15/9/31605, PG/14/59/31000, RG/14/1/30588, RM/13/30157, P47352/CRMMedical Research Council MR/M017354/1National Centre for the Replacement, Refinement and Reduction of Animals in Research NC/C013105/1National Centre for the Replacement, Refinement and Reduction of Animals in Research NC/C013202/1National Centre for the Replacement, Refinement and Reduction of Animals in Research NC/K000225/1National Centre for the Replacement, Refinement and Reduction of Animals in Research NC/S001808/1
6 · The paper itself

Abstract

Twenty years since their first derivation, human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) have shown promise in disease modelling research, while their potential for cardiac repair is being investigated. However, low transfection efficiency is a barrier to wider realisation of the potential this model system has to offer. We endeavoured to produce a protocol for improved transfection of hPSC-CMs using the Viafect

Indexed as

disease modellinghuman pluripotent stem cell-derived cardiomyocytestransfectionViafectTM

Identifiers

PMID32784848
PMCPMC7564709
OpenAlexW3048126678

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.