Evidence map›Paper›PMID 32784770›Full record

ArticleInternational journal of molecular sciences2020

Development and Evaluation of Novel and Highly Sensitive Single-Tube Nested Real-Time RT-PCR Assays for SARS-CoV-2 Detection.

Cyril Chik-Yan Yip, Siddharth Sridhar, Kit-Hang Leung, Anthony Chin-Ki Ng, Kwok-Hung Chan, Jasper Fuk-Woo Chan, Owen Tak-Yin Tsang, Ivan Fan-Ngai Hung, Vincent Chi-Chung Cheng, Kwok-Yung Yuen and 1 more

Open access · goldAbstract readEvaluation Study
In one paragraph

Article in International journal of molecular sciences, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed, 2 pooled it
0.6field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 2 syntheses or guidelines pooled it, 26 citations in OpenAlex.

  1. Pooled it
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  5. Review
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  7. Intravenous Injection of Coronavirus Disease 2019 (COVID-19) mRNA Vaccine Can Induce Acute Myopericarditis in Mouse Model.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2022
    Article
  8. Article
  9. Review
  10. Development of a genotyping platform for SARS-CoV-2 variants using high-resolution melting analysis.Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy · 2021
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 2 countries.

Cyril Chik-Yan YipDepartment of Microbiology, Queen Mary Hospital, Hong Kong, China.
Siddharth SridharDepartment of Microbiology, The University of Hong Kong, Hong Kong, China.ORCID 0000-0002-2022-8307
Kit-Hang LeungDepartment of Microbiology, The University of Hong Kong, Hong Kong, China.
Anthony Chin-Ki NgDepartment of Microbiology, The University of Hong Kong, Hong Kong, China.
Kwok-Hung ChanDepartment of Microbiology, The University of Hong Kong, Hong Kong, China.
Jasper Fuk-Woo ChanDepartment of Microbiology, The University of Hong Kong, Hong Kong, China.ORCID 0000-0001-6336-6657
Owen Tak-Yin TsangDepartment of Medicine and Geriatrics, Princess Margaret Hospital, Hong Kong, China.
Ivan Fan-Ngai HungDepartment of Medicine, The University of Hong Kong, Hong Kong, China.
Vincent Chi-Chung ChengDepartment of Microbiology, Queen Mary Hospital, Hong Kong, China.
Kwok-Yung YuenDepartment of Microbiology, Queen Mary Hospital, Hong Kong, China.
Kelvin Kai-Wang ToDepartment of Microbiology, The University of Hong Kong, Hong Kong, China.
University of Hong Kong · HKQueen Mary Hospital · CN

Funding

the Consultancy Service for Enhancing Laboratory Surveillance of Emerging Infectious Diseases and Research Capability on Antimicrobial Resistance for Department of Health of the Hong Kong Special Administrative Region Government NAthe donations of Richard Yu and Carol Yu, the Shaw Foundation Hong Kong, May Tam Mak Mei Yin, Michael Seak-Kan Tong, Respiratory Viral Research Foundation Limited, Hui Ming, Hui Hoy and Chow Sin Lan Charity Fund Limited, Chan Yin Chuen Memorial Charitable NAthe Theme-Based Research Scheme of the Research Grants Council, Hong Kong Special Administrative Region T11/707/15
6 · The paper itself

Abstract

Sensitive molecular assays are critical for coronavirus disease 2019 (COVID-19) diagnosis. Here, we designed and evaluated two single-tube nested (STN) real-time RT-PCR assays, targeting SARS-CoV-2 RdRp/Hel and

Indexed as

BetacoronavirusCoronavirus InfectionsCoronavirus Nucleocapsid ProteinsCoronavirus RNA-Dependent RNA PolymeraseCOVID-19HumansMolecular Diagnostic TechniquesNucleocapsid ProteinsPandemicsPhosphoproteinsPneumonia, ViralReal-Time Polymerase Chain ReactionRespiratory MucosaRNA-Dependent RNA PolymeraseSARS-CoV-2Sensitivity and SpecificityCoronavirus Nucleocapsid ProteinsCoronavirus RNA-Dependent RNA PolymeraseNSP12 protein, SARS-CoV-2nucleocapsid phosphoprotein, SARS-CoV-2Nucleocapsid ProteinsPhosphoproteinsRNA-Dependent RNA PolymeraseViral Nonstructural ProteinsCOVID-19evaluationRT-PCRSARS-CoV-2single-tube nested

Identifiers

PMID32784770
PMCPMC7461039
OpenAlexW3048140003

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.