Synthesis and Initial Evaluation of a Novel Fluorophore for Selective FMDV 3C Protease Detection.

Samerah Malik, Alex Sinclair, Ali Ryan, Adam Le Gresley

Open access · goldAbstract read

In one paragraph

Article in Molecules (Basel, Switzerland), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact, top 84% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Samerah MalikChemical and Pharmaceutical Sciences, SEC Faculty, Kingston University, Kingston-upon-Thames, London KT1 2EE, UK.

Alex SinclairChemical and Pharmaceutical Sciences, SEC Faculty, Kingston University, Kingston-upon-Thames, London KT1 2EE, UK.

Ali RyanChemical and Pharmaceutical Sciences, SEC Faculty, Kingston University, Kingston-upon-Thames, London KT1 2EE, UK.ORCID 0000-0002-4627-8185

Adam Le GresleyChemical and Pharmaceutical Sciences, SEC Faculty, Kingston University, Kingston-upon-Thames, London KT1 2EE, UK.

Kingston University · GB

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The development and evaluation of a Boc-AL(Boc)Q(Trt)-AMC fluorophore to detect 3C Protease, produced by Foot and Mouth Disease Virus (FMDV) is reported, with a view to a potential use as a rapid screen for FMDV infected livestock The peptide-linked conjugate fluorophore is evaluated in vitro for sensitivity, specificity, stability and rapidity and shows statistically significant increases in fluorescence when exposed to physiologically relevant concentrations of 3C Protease and selectivity when compared with other common proteases likely to be located, typically in the absence of FMDV. The stability of deprotected Boc-AL(Boc)Q(Trt)-AMC is reported as a limitation of this probe.

Indexed as

3C Viral ProteasesCell LineChemistry Techniques, SyntheticCysteine EndopeptidasesEnzyme AssaysFluorescent DyesFoot-and-Mouth Disease VirusViral Proteins3C Viral ProteasesCysteine EndopeptidasesFluorescent DyesViral Proteins3C proteasefluorescenceFMDVveterinary testing

Identifiers

PMID32784761

DOI10.3390/molecules25163599

PMCPMC7465021

OpenAlexW3048321077

What OpenQuestion holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.