ArticleBiomedicines2020
How Long Does a Neutrophil Live?-The Effect of 24 h Whole Blood Storage on Neutrophil Functions in Pigs.
Article in Biomedicines, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 35 citations in OpenAlex.
- Single-cell transcriptomics provides insights into the stress-immune interplay and informs disease risk.Cell genomics · 2026Article
- Beyond the Human Body: Models for Studying Neutrophil Biology.Immunology and cell biology · 2026Review
- Immunomodulatory effect of stress hormones on porcine neutrophil functions during Actinobacillus pleuropneumoniae infection.Medical microbiology and immunology · 2026Article
- Differential Neutrophil Responses to Smooth Titanium: A Double-Edged Sword for the Abutment-Gingival Interface (An In Vitro Study).International journal of dentistry · 2026Article
- Stomoxys calcitrans as a potential mechanical vector of Anaplasma phagocytophilum: assessment through original ex vivo feeding models.Parasite (Paris, France) · 2026Article
- Neutrophil extracellular traps: the hidden driver of gastrointestinal cancer and emerging therapeutic strategies.Frontiers in immunology · 2026Review
- Immune cell response after intracerebral hemorrhage in piglets and the treatment effects of deferoxamine and minocycline.Experimental neurology · 2025Article
- Impact of pre-analytical variables - temperature, agitation, storage duration, and blood-to-anticoagulant ratio - on complete blood count test reliability.Medicine and pharmacy reports · 2025Article
- Comparison of single-cell RNA-seq methods to enable transcriptome profiling of neutrophils in clinical samples.Cell reports methods · 2025Article
- Transdifferentiation of neutrophils facilitates the establishment of infection byInfection and immunity · 2025Article
- A Web of Challenges: The Therapeutic Struggle to Target NETs in Disease.International journal of molecular sciences · 2025Review
- Tapping natures rhythm: the role of season in mitochondrial function and genetics in the UK biobank.Human genomics · 2025Article
- Role of Neutrophils as Therapeutic Targets in Intracerebral Hemorrhage.Therapeutic innovation & regulatory science · 2024Review
- Human Macrophages Activate Bystander Neutrophils' Metabolism and Effector Functions When Challenged withInternational journal of molecular sciences · 2024Article
- The effect of whole blood logistics on neutrophil non-specific activation and kinetics ex vivo.Scientific reports · 2024Article
- Article
- Encapsulated Streptococcus suis impairs optimal neutrophil functions which are not rescued by priming with colony-stimulating factors.PloS one · 2024Article
- A Comparative Study of Different Protocols for Isolation of Murine Neutrophils from Bone Marrow and Spleen.International journal of molecular sciences · 2023Article
- Hematologic Differences Between Migraineurs and Tension-Type Headache Patients.Medical archives (Sarajevo, Bosnia and Herzegovina) · 2023Article
- New Insights into Neutrophil Extracellular Trap (NETs) Formation from Porcine Neutrophils in Response to Bacterial Infections.International journal of molecular sciences · 2022Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 2 countries.
Funding
Abstract
Neutrophils are important effector cells of the innate immune system, traditionally regarded to have a short life span. The goal of this study was to evaluate the effect of the whole blood storage on neutrophil functions, e.g., viability, antimicrobial effect, neutrophil extracellular trap (NET) formation and phagocytosis. Therefore, fresh porcine whole blood was compared to whole blood stored for 24 h in the dark at room temperature. Different cell parameters in whole blood and in isolated neutrophils were analyzed. The following parameters were analyzed: cell count, band and segmented neutrophil count, viability, cholesterol content, release of free DNA as a marker for cell death, phagocytic activity in whole blood and in isolated neutrophils, the transmigration rate of neutrophils to IL8 stimulus, the production of reactive oxygen species (ROS), and the formation of NETs. It was observed that the number of isolated neutrophils decreased over time, indicating cell death occurs during 24 h of blood storage. However, the surviving neutrophils isolated from stored blood reacted comparably or even showed enhanced antimicrobial activity in the case of phagocytosis of
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.