ArticleThe Science of the total environment2020
First environmental surveillance for the presence of SARS-CoV-2 RNA in wastewater and river water in Japan.
Article in The Science of the total environment, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 322 papers, 3 of them syntheses that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
322 citing papers in PubMed, 3 syntheses or guidelines pooled it, 600 citations in OpenAlex.
- A Systematic Review of Methodological Approaches to SARS-CoV-2 Wastewater Surveillance.Viruses · 2026Pooled it
- Recent progress on wastewater-based epidemiology for COVID-19 surveillance: A systematic review of analytical procedures and epidemiological modeling.The Science of the total environment · 2023Pooled it
- Wastewater surveillance to infer COVID-19 transmission: A systematic review.The Science of the total environment · 2022Pooled it
- Co-prevalence of SARS-CoV-2 and Noroviruses in Wastewater Collection and Treatment Facilities of the Eastern Cape Region, South Africa: An Exacerbated Public and Environmental Health Risk Concern.Food and environmental virology · 2026Article
- Long-term biological surveillance of SARS-CoV-2 in critical points for municipal sewage catchment in light of wastewater-based epidemiology, public health and environmental hygiene.Scientific reports · 2025Article
- Genomic fragment detection and infectivity evaluation of rotaviruses isolated from wastewater used for irrigation in western Bogotá, D. C.Biomedica : revista del Instituto Nacional de Salud · 2025Article
- Wastewater Surveillance of Enteroviruses Potentially Associated with Hand, Foot, and Mouth Disease in Nepal.Food and environmental virology · 2025Article
- Noncompetitive Fluorescence Polarization Aptamer Assay for Detecting SARS-CoV‑2 Variants.ACS omega · 2025Article
- Wastewater-Based Tracking SARS-CoV-2 RNA and Estimating Infected Population in Industrial Setting After Songkran Festival-Thailand Traditional New Year in 2023.Food and environmental virology · 2025Article
- Long term assessment of SARS-CoV-2 in wastewater and the transition to evaluate additional viral targets.The Science of the total environment · 2025Article
- Integrating wastewater surveillance for future pandemic preparedness by tracking SARS-CoV-2 RNA in municipal wastewater in Southwest Iran.Scientific reports · 2025Article
- Assess the Variability and Robustness of an Aluminum-Based Adsorption-Precipitation Method for Virus Detection in Wastewater Samples.Microorganisms · 2025Article
- Measuring SARS-CoV-2 RNA in Bangkok wastewater treatment plants and estimating infected population after fully opening the country in 2023, Thailand.Scientific reports · 2025Article
- Hydrological and physicochemical parameters associated with SARS-CoV-2 and pepper mild mottle virus wastewater concentrations for a large-combined sewer system.Journal of water and health · 2025Article
- Environmental Dissemination of SARS-CoV-2: An Analysis Employing Crassphage and Next-Generation Sequencing Protocols.Food and environmental virology · 2025Article
- Cost-effectiveness of wastewater-based environmental surveillance for SARS-CoV-2 in Blantyre, Malawi and Kathmandu, Nepal: A model-based study.PLOS global public health · 2025Article
- A Narrative Review of High Throughput Wastewater Sample Processing for Infectious Disease Surveillance: Challenges, Progress, and Future Opportunities.International journal of environmental research and public health · 2024Review
- The first detection of SARS-CoV-2 RNA in the wastewater of Bucharest, Romania.Scientific reports · 2024Article
- Seasonal Prevalence and Detection of Enteric and Respiratory Viruses in Wastewater and Hospitalized Children with Acute Gastroenteritis.Current microbiology · 2024Article
- Inter-institutional laboratory standardization for SARS-CoV-2 surveillance through wastewater-based epidemiology applied to Mexico City.IJID regions · 2024Article
262 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Wastewater-based epidemiology is a powerful tool to understand the actual incidence of coronavirus disease 2019 (COVID-19) in a community because severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the etiological agent of COVID-19, can be shed in the feces of infected individuals regardless of their symptoms. The present study aimed to assess the presence of SARS-CoV-2 RNA in wastewater and river water in Yamanashi Prefecture, Japan, using four quantitative and two nested PCR assays. Influent and secondary-treated (before chlorination) wastewater samples and river water samples were collected five times from a wastewater treatment plant and three times from a river, respectively, between March 17 and May 7, 2020. The wastewater and river water samples (200-5000 mL) were processed by using two different methods: the electronegative membrane-vortex (EMV) method and the membrane adsorption-direct RNA extraction method. Based on the observed concentrations of indigenous pepper mild mottle virus RNA, the EMV method was found superior to the membrane adsorption-direct RNA extraction method. SARS-CoV-2 RNA was successfully detected in one of five secondary-treated wastewater samples with a concentration of 2.4 × 10
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.