Evidence map›Paper›PMID 32782716›Full record

ArticleInternational journal of clinical and experimental pathology2020

Alveolar macrophages in patients with non-small cell lung cancer.

Jinfeng Li, Jinlong Li, Lingxiong Wang, Jianqing Hao, Liangliang Wu, Haitao Tao, Sujie Zhang, Pengfei Cui, Xiaoyan Li, Xiaoyun Zhang and 3 more

Abstract read
PubMed Full text at PMC
In one paragraph

Article in International journal of clinical and experimental pathology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Jinfeng LiDepartment of Oncology, First Department of Medical Oncology, Chinese PLA General Hospital Beijing, China.
Jinlong LiDepartment of Pathology, First Department of Medical Oncology, Chinese PLA General Hospital Beijing, China.
Lingxiong WangDepartment of Oncology, First Department of Medical Oncology, Chinese PLA General Hospital Beijing, China.
Jianqing HaoQingyang People's Hospital Qingyang, Gansu Province, China.
Liangliang WuDepartment of Oncology, First Department of Medical Oncology, Chinese PLA General Hospital Beijing, China.
Haitao TaoDepartment of Oncology, First Department of Medical Oncology, Chinese PLA General Hospital Beijing, China.
Sujie ZhangDepartment of Oncology, First Department of Medical Oncology, Chinese PLA General Hospital Beijing, China.
Pengfei CuiDepartment of Oncology, First Department of Medical Oncology, Chinese PLA General Hospital Beijing, China.
Xiaoyan LiDepartment of Oncology, First Department of Medical Oncology, Chinese PLA General Hospital Beijing, China.
Xiaoyun ZhangDepartment of Oncology, First Department of Medical Oncology, Chinese PLA General Hospital Beijing, China.
Zhou ZhouDepartment of Oncology, First Department of Medical Oncology, Chinese PLA General Hospital Beijing, China.
Shunchang JiaoDepartment of Oncology, First Department of Medical Oncology, Chinese PLA General Hospital Beijing, China.
Yi HuDepartment of Oncology, First Department of Medical Oncology, Chinese PLA General Hospital Beijing, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveTo observe alveolar macrophages (AMs) in the microenvironment of patients with non-small cell lung cancer (NSCLC). MATERIALS AND

methods20 NSCLC patients received bronchoalveolar lavage, and the bronchial alveolar lavage fluid (BALF) was collected. The phenotypes of AMs were detected by the opal multiplex immunofluorescence assay (mIF), flow cytometry, and western blot.

resultsAMs could easily be made into paraffin sections after agar pre-embedding. The mIF results showed that AMs highly expressed M1-type marker CD86, and M2-type marker CD163 under PerkinElmer Vectra microscope, while there was a significant difference between the expression of CD86 and CD163 (**

conclusionsOur results showed that AMs simultaneously expressed M1-type markers and M2-type markers, while the M2 markers still dominated. This suggests agar pre-embedding is a very convenient method to embed cells to paraffin tissue, so that cell membrane or nuclear antigens are very easily detected by mIF.

Indexed as

alveolar macrophagesbroncho-lavage alveolar fluidNon-small cell lung canceropal multiplex immunofluorescence assay

Identifiers

PMID32782716
PMCPMC7414454

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