Evidence map›Paper›PMID 32782555›Full record

ArticleOncology letters2020

Bioinformatics analysis of aberrantly expressed exosomal lncRNAs in oral squamous cell carcinoma (CAL-27 vs. oral epithelial) cells.

Jiajia Jin, Zixiao Huang, Xiaoyan Lu, Shengrong Wu, Mei'E Jia, Xin Li, Zhiyong Li, Xiangyi He

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Oncology letters, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.2field-weighted citation impact, top 57% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 5 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Jiajia JinDepartment of Prosthodontics, School of Dentistry, Lanzhou University, Lanzhou, Gansu 730000, P.R. China.
Zixiao HuangDepartment of Prosthodontics, School of Dentistry, Lanzhou University, Lanzhou, Gansu 730000, P.R. China.
Xiaoyan LuDepartment of Prosthodontics, School of Dentistry, Lanzhou University, Lanzhou, Gansu 730000, P.R. China.
Shengrong WuDepartment of Prosthodontics, School of Dentistry, Lanzhou University, Lanzhou, Gansu 730000, P.R. China.
Mei'E JiaDepartment of Prosthodontics, School of Dentistry, Lanzhou University, Lanzhou, Gansu 730000, P.R. China.
Xin LiDepartment of Prosthodontics, School of Dentistry, Lanzhou University, Lanzhou, Gansu 730000, P.R. China.
Zhiyong LiState Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu 730046, P.R. China.
Xiangyi HeDepartment of Prosthodontics, School of Dentistry, Lanzhou University, Lanzhou, Gansu 730000, P.R. China.
Lanzhou University · CNLanzhou Veterinary Research Institute · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Oral squamous cell carcinoma (OSCC) is the most prevalent form of malignant tumour in the oral cavity and its early detection is critical for improving the prognosis of affected patients. The present study aimed to isolate and confirm exosomes derived from the supernatant of the OSCC cell line CAL-27 and human oral epithelial cells (HOECs), analyze long non-coding RNA (lncRNA) expression profiles and determine the diagnostic value based on bioinformatics analyses. The results indicated that the particles isolated from the supernatant of CAL-27 and HOECs were either round or oval, had a size range of 30-150 nm and were enriched with ALG-2 interacting protein X (ALIX) and tumour susceptibility 101 proteins (TSG101). These characteristics confirmed that these particles were exosomes. Three lncRNAs (NR-026892.1, NR-126435.1 and NR-036586.1) were selected as potential diagnostic biomarkers for OSCC. The expression levels of the selected lncRNAs were significantly different in CAL-27-exo vs. HOEC-exo, as well as in whole cells (CAL-27 vs. HOECs) (P<0.001). The expression levels of the three lncRNAs confirmed by quantitative PCR were consistent with the sequencing data. In conclusion, various lncRNAs were aberrantly expressed between cancerous and non-cancerous exosomes, suggesting that they may serve as biomarkers for cancer.

Indexed as

bioinformaticsexosomelong non-coding RNAsoral squamous cell carcinoma

Identifiers

PMID32782555
PMCPMC7400702
OpenAlexW3037412241

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.