Evidence map›Paper›PMID 32782408›Full record

ArticleNature plants2020

A high-contiguity Brassica nigra genome localizes active centromeres and defines the ancestral Brassica genome.

Sampath Perumal, Chu Shin Koh, Lingling Jin, Miles Buchwaldt, Erin E Higgins, Chunfang Zheng, David Sankoff, Stephen J Robinson, Sateesh Kagale, Zahra-Katy Navabi and 7 more

Open access · hybridAbstract read
In one paragraph

Article in Nature plants, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 88 papers.

0numbers the graph read from it
0cells of the map it votes in
88citing papers in PubMed
26.0field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

88 citing papers in PubMed, 166 citations in OpenAlex.

  1. Article
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  4. Segregating BCMolecular breeding : new strategies in plant improvement · 2026
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  10. Genome-Wide Identification of theInternational journal of molecular sciences · 2026
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  20. Development of Brassica rapa-nigra monosomic alien addition lines: cytology, genetics, and morphology.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025
    Article

28 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors at 7 institutions in 3 countries.

Sampath PerumalAgriculture and Agri-Food Canada, Saskatoon, Saskatchewan, Canada.
Chu Shin KohGlobal Institute for Food Security, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
Lingling JinDepartment of Computing Science, Thompson Rivers University, Kamloops, British Columbia, Canada.
Miles BuchwaldtAgriculture and Agri-Food Canada, Saskatoon, Saskatchewan, Canada.
Erin E HigginsAgriculture and Agri-Food Canada, Saskatoon, Saskatchewan, Canada.
Chunfang ZhengDepartment of Mathematics and Statistics, University of Ottawa, Ottawa, Ontario, Canada.
David SankoffDepartment of Mathematics and Statistics, University of Ottawa, Ottawa, Ontario, Canada.ORCID http://orcid.org/0000-0001-8415-5189
Stephen J RobinsonAgriculture and Agri-Food Canada, Saskatoon, Saskatchewan, Canada.
Sateesh KagaleNational Research Council Canada, Saskatoon, Saskatchewan, Canada.ORCID http://orcid.org/0000-0002-7213-1590
Zahra-Katy NavabiAgriculture and Agri-Food Canada, Saskatoon, Saskatchewan, Canada.
Lily TangAgriculture and Agri-Food Canada, Saskatoon, Saskatchewan, Canada.
Kyla N HornerAgriculture and Agri-Food Canada, Saskatoon, Saskatchewan, Canada.
Zhesi HeDepartment of Biology, University of York, York, UK.ORCID http://orcid.org/0000-0001-8335-9876
Ian BancroftDepartment of Biology, University of York, York, UK.ORCID http://orcid.org/0000-0001-7707-1171
Boulos ChalhoubInstitute of Crop Science, Zhejiang University, Hangzhou, China.
Andrew G SharpeGlobal Institute for Food Security, University of Saskatchewan, Saskatoon, Saskatchewan, Canada. andrew.sharpe@gifs.ca.ORCID http://orcid.org/0000-0002-1832-4009
Isobel A P ParkinAgriculture and Agri-Food Canada, Saskatoon, Saskatchewan, Canada. isobel.parkin@canada.ca.ORCID http://orcid.org/0000-0002-5807-9466
Agriculture and Agri-Food Canada · CAUniversity of Ottawa · CAUniversity of Saskatchewan · CAUniversity of York · GBSaskatchewan Research Council (Canada) · CAThompson Rivers University · CAZhejiang University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

It is only recently, with the advent of long-read sequencing technologies, that we are beginning to uncover previously uncharted regions of complex and inherently recursive plant genomes. To comprehensively study and exploit the genome of the neglected oilseed Brassica nigra, we generated two high-quality nanopore de novo genome assemblies. The N50 contig lengths for the two assemblies were 17.1 Mb (12 contigs), one of the best among 324 sequenced plant genomes, and 0.29 Mb (424 contigs), respectively, reflecting recent improvements in the technology. Comparison with a de novo short-read assembly corroborated genome integrity and quantified sequence-related error rates (0.2%). The contiguity and coverage allowed unprecedented access to low-complexity regions of the genome. Pericentromeric regions and coincidence of hypomethylation enabled localization of active centromeres and identified centromere-associated ALE family retro-elements that appear to have proliferated through relatively recent nested transposition events (<1 Ma). Genomic distances calculated based on synteny relationships were used to define a post-triplication Brassica-specific ancestral genome, and to calculate the extensive rearrangements that define the evolutionary distance separating B. nigra from its diploid relatives.

Indexed as

BrassicaCentromereDNA, PlantEvolution, MolecularGenome, PlantHigh-Throughput Nucleotide SequencingMustard PlantDNA, Plant

Identifiers

PMID32782408
PMCPMC7419231
OpenAlexW3047959744

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.