ArticleScientific reports2020
Effect of manuka honey on biofilm-associated genes expression during methicillin-resistant Staphylococcus aureus biofilm formation.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 40 citations in OpenAlex.
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- Network-based bioinformatics for the prediction of candidate SNPs in Staphylococcus aureus virulence and resistance genes.Folia microbiologica · 2026Article
- Caffeic Acid Phenethyl Ester Suppresses Adhesion to Mediate Its Antibiofilm Activity Against Methicillin-ResistantMicroorganisms · 2026Article
- Efficiency of NSAIDs in preventing prosthetic joint infections (PJIs): inhibition of Staphylococcus aureus biofilms by etoricoxib through down regulation of bacterial adhesion genes.Archives of microbiology · 2026Article
- A Korean native halophyte extract attenuates the virulence of methicillin-resistant Staphylococcus aureus by inhibiting biofilm formation.Scientific reports · 2026Article
- Potential of Jelleine-I peptide on down-regulation of biofilm-associated genes and the biofilm formation of methicillin-resistant Staphylococcus aureus.Molecular biology reports · 2026Article
- Propolis Exerts Antibiofilm Activity Against Methicillin-ResistantMicroorganisms · 2025Article
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- Proteomic analysis reveals phage-driven metabolic shifts and biofilm disruption in methicillin-resistant Staphylococcus aureus (MRSA).World journal of microbiology & biotechnology · 2025Article
- Identification of metabolic pathways modulated by GAM and NGAM in the inhibition ofFrontiers in microbiology · 2025Article
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- Evaluation ofArchives of Razi Institute · 2024Article
- The wound microbiota: microbial mechanisms of impaired wound healing and infection.Nature reviews. Microbiology · 2024Review
- Bioactive Dressing: A New Algorithm in Wound Healing.Journal of clinical medicine · 2024Review
- Manuka honey as a non-antibiotic alternative againstFrontiers in cellular and infection microbiology · 2024Review
- Transcriptomic analysis of cell envelope inhibition by prodigiosin in methicillin-resistantFrontiers in microbiology · 2024Article
- Evaluation of Virulence, Antimicrobial Resistance and Biofilm Forming Potential of Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates from Bovine Suspected with Mastitis.Current microbiology · 2023Article
- Biofilm and wound healing: from bench to bedside.European journal of medical research · 2023Review
- Effect of Savirin or Ticagrelor Treatment on the Expression of Commonly Used Reference Genes inMicroorganisms · 2023Article
- Evaluation of the polyphenolic profile of native Ecuadorian stingless bee honeys (Current research in food science · 2023Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Methicillin-resistant Staphylococcus aureus (MRSA) are among the most important biofilm-forming pathogens responsible for hard-to-treat infections. Looking for alternatives to antibiotics that prevent biofilm formation, we investigated the effects of manuka honey on the transcriptional profile of genes essential for staphylococcal biofilm formation using qRT-PCR. mRNA from two hospital MRSA strains (strong and weak biofilm producer) were isolated after 4, 8, 12 and 24 h from cells grown in biofilm. Manuka honey at 1/2 minimum biofilm inhibition concentration (MBIC) significantly reduced MRSA cell viability in biofilm. Manuka honey downregulated the genes encoding laminin- (eno), elastin- (ebps) and fibrinogen binding protein (fib), and icaA and icaD involved in biosynthesis of polysaccharide intercellular adhesin in both weakly and strongly adhering strain compared to the control (untreated biofilm). Expression levels of cna (collagen binding protein) and map/eap (extracellular adherence protein-Eap) were reduced in weakly adhering strain. The lowest expression of investigated genes was observed after 12 h of manuka honey treatment at 1/2 MBIC. This study showed that the previously unknown mechanism of manuka honey action involved inhibition of S. aureus adhesion due to reduction in expression of crucial genes associated with staphylococcal biofilm.
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