ArticleScientific data2020
A comprehensive CHO SWATH-MS spectral library for robust quantitative profiling of 10,000 proteins.
Article in Scientific data, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 36 citations in OpenAlex.
- Improving recombinant protein productivity in CHO cells via multi-omics data integration.Bioresources and bioprocessing · 2026Review
- Host cell protein impurities in therapeutic proteins: overview of advances in detection, nonconventional removal technologies and immunogenicity assessment.Journal of biological engineering · 2026Review
- A reference database enabling in-depth proteome and PTM analysis of mouse immune cells.Scientific data · 2025Article
- Grape-Pi: graph-based neural networks for enhanced protein identification in proteomics pipelines.Bioinformatics advances · 2025Article
- SWATH-MS insights on sodium butyrate effect on mAbs production and redox homeostasis in CHO cells.AMB Express · 2024Article
- Temporal insights into molecular and cellular responses during rAAV production in HEK293T cells.Molecular therapy. Methods & clinical development · 2024Article
- A crustacean neuropeptide spectral library for data-independent acquisition (DIA) mass spectrometry applications.Proteomics · 2024Article
- Identification and characterization of CHO host-cell proteins in monoclonal antibody bioprocessing.Biotechnology and bioengineering · 2024Article
- Collision Induced Unfolding Enables the Quantitation of Isomass Biotherapeutics in Complex Biological Matrices.Journal of the American Society for Mass Spectrometry · 2023Article
- Systematic Adaptation of Bacillus licheniformis to 2-Phenylethanol Stress.Applied and environmental microbiology · 2023Article
- Cottonseed Meal Protein Isolate as a New Source of Alternative Proteins: A Proteomics Perspective.International journal of molecular sciences · 2022Article
- Metaproteomics insights into fermented fish and vegetable products and associated microbes.Food chemistry. Molecular sciences · 2021Review
- Narrow Precursor Mass Range for DIA-MS Enhances Protein Identification and Quantification in Arabidopsis.Life (Basel, Switzerland) · 2021Article
- A comprehensive CHO SWATH-MS spectral library for robust quantitative profiling of 10,000 proteins.Scientific data · 2020Article
- Review
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sequential window acquisition of all theoretical fragment-ion spectra (SWATH) is a data-independent acquisition (DIA) strategy that requires a specific spectral library to generate unbiased and consistent quantitative data matrices of all peptides. SWATH-MS is a promising approach for in-depth proteomic profiling of Chinese hamster Ovary (CHO) cell lines, improving mechanistic understanding of process optimization, and real-time monitoring of process parameters in biologics R&D and manufacturing. However, no spectral library for CHO cells is publicly available. Here we present a comprehensive CHO global spectral library to measure the abundance of more than 10,000 proteins consisting of 199,102 identified peptides from a CHO-K1 cell proteome. The robustness, accuracy and consistency of the spectral library were validated for high confidence in protein identification and reproducible quantification in different CHO-derived cell lines, instrumental setups and downstream processing samples. The availability of a comprehensive SWATH CHO global spectral library will facilitate detailed characterization of upstream and downstream processes, as well as quality by design (QbD) in biomanufacturing. The data have been deposited to ProteomeXchange (PXD016047).
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