ArticleMaterials (Basel, Switzerland)2020
Liquid Platelet-Rich Fibrin and Heat-Coagulated Albumin Gel: Bioassays for TGF-β Activity.
Article in Materials (Basel, Switzerland), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 28 citations in OpenAlex.
- Effects of albumin-platelet-rich fibrin as an adjunct to non-surgical periodontal therapy in patients with periodontitis: a randomized controlled clinical trial.BMC oral health · 2026Trial
- Comparison of root coverage by injectable-platelet rich fibrin in thick and thin phenotypes: a parallel-armed, prospective, preliminary clinical study with up to 48 months of follow-up.Journal of applied oral science : revista FOB · 2026Trial
- Clinical effectiveness of albumin-enriched platelet-rich fibrin (Alb-PRF) in periodontal and peri-implant regenerative surgery: a systematic review.Odontology · 2026Review
- Impact of Thermal Treatment on Growth Factor Levels in Injectable Horizontal Platelet-Rich Fibrin: An In Vitro Study.Clinical and experimental dental research · 2026Article
- Special Issue "Recent Progress in Regenerative Therapy Using Blood-Derived Biomaterials".International journal of molecular sciences · 2026Article
- Advantages of horizontal centrifugation of platelet-rich fibrin in regenerative medicine and dentistry.Periodontology 2000 · 2025Review
- Extended PRF: Impact of Heat on Gene Expression in Gingival Fibroblasts.International journal of molecular sciences · 2025Article
- Efficacy of platelet-rich albumin and fibrin gel in two-stage lateral sinus lift: a prospective study.Annals of medicine and surgery (2012) · 2025Article
- A comparative study of three liquid platelet concentrates on human primary osteoblast activity: an in vitro study.Journal of applied oral science : revista FOB · 2025Article
- Article
- Human versus Rat PRF on Collagen Membranes: A Pilot Study of Mineralization in Rat Calvaria Defect Model.Bioengineering (Basel, Switzerland) · 2024Article
- Plasma Gel Matrix as a Promising Carrier of Epigallocatechin Gallate for Regenerative Medicine.Journal of functional biomaterials · 2024Article
- Effect of thermal manipulation on the biological and mechanical characteristics of horizontal platelet rich fibrin membranes.BMC oral health · 2023Article
- Gingival Fibroblasts Are Sensitive to Oral Cell Lysates Indicated by Their IL11 Expression.Bioengineering (Basel, Switzerland) · 2023Article
- Clinical application of platelet-rich fibrin to enhance dental implant stability: A systematic review and meta-analysis.Heliyon · 2023Article
- Exploration of proper heating protocol for injectable horizontal platelet-rich fibrin gel.International journal of implant dentistry · 2022Article
- Blood Clots versus PRF: Activating TGF-β Signaling and Inhibiting Inflammation In Vitro.International journal of molecular sciences · 2022Article
- The feasibility of injectable PRF (I-PRF) for bone tissue engineering and its application in oral and maxillofacial reconstruction: From bench to chairside.Biomaterials advances · 2022Review
- RNAseq of TGF-β receptor type I kinase-dependent genes in oral fibroblast exposed to milk.BMC oral health · 2021Article
- Platelet-Rich Fibrin Increases BMP2 Expression in Oral Fibroblasts via Activation of TGF-β Signaling.International journal of molecular sciences · 2021Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Liquid platelet-rich fibrin (PRF) can be prepared by high centrifugation forces separating the blood into a platelet-poor plasma (PPP) layer and a cell-rich buffy coat layer, termed concentrated PRF (C-PRF). Heating the liquid PPP was recently introduced to prepare an albumin gel (Alb-gel) that is later mixed back with the concentrated liquid C-PRF to generate Alb-PRF. PRF is a rich source of TGF-β activity; however, the overall TGF-β activity in the PPP and the impact of heating the upper plasma layer remains unknown. Here, we investigated for the first time the in vitro TGF-β activity of all fractions of Alb-PRF. We report that exposure of oral fibroblasts with lysates of PPP and the buffy coat layer, but not with heated PPP, provoked a robust increase in the TGF-β target genes interleukin 11 and NADPH oxidase 4 by RT-PCR, and for IL11 by immunoassay. Consistent with the activation of TGF-β signaling, expression changes were blocked in the presence of the TGF-β receptor type I kinase inhibitor SB431542. Immunofluorescence and Western blot further confirmed that lysates of PPP and the buffy coat layer, but not heated PPP, induced the nuclear translocation of Smad2/3 and increased phosphorylation of Smad3. The immunoassay further revealed that PPP and particularly BC are rich in active TGF-β compared to heated PPP. These results strengthen the evidence that not only the cell-rich C-PRF but also PPP comprise a TGF-β activity that is, however, heat sensitive. It thus seems relevant to mix the heated PPP with the buffy coat C-PRF layer to regain TGF-β activity, as proposed during the preparation of Alb-PRF.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.