ReviewProteomes2020
What Room for Two-Dimensional Gel-Based Proteomics in a Shotgun Proteomics World?
Review in Proteomes, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
40 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Studying the Impact of Persistent Organic Pollutants Exposure on Human Health by Proteomic Analysis: A Systematic Review.International journal of molecular sciences · 2022Pooled it
- Retinal proteomics in neurodegeneration: Insights into ocular and brain disorders.Neural regeneration research · 2026Article
- Proteomic Profiling and Targeted Validation Reveal a More Extensive Salivary than Serum Response to a Feed-Deprivation Protocol in Horses with Gastric Lesions.Animals : an open access journal from MDPI · 2026Article
- Clinical Proteomics Applied to Food Allergy.Advances in experimental medicine and biology · 2026Review
- Contribution of proteomics and metabolomics data to understanding of health benefits of tea.Chinese herbal medicines · 2026Review
- Regional profiling reveals a distinct glioblastoma infiltrative margin proteome.Scientific reports · 2025Article
- Proteomic Analysis and Expression of Selected Genes During the Early Somatic Embryogenesis ofInternational journal of molecular sciences · 2025Article
- Article
- Plasma proteomics in pediatric patients with sepsis- hopes and challenges.Clinical proteomics · 2025Review
- Multi-omics approaches for biomarker discovery and precision diagnosis of prediabetes.Frontiers in endocrinology · 2025Review
- Betaine supplementation affects carbohydrate metabolism in the skeletal muscle of finishing pigs.PloS one · 2025Article
- Evaluation of the Protein Profile of aFoods (Basel, Switzerland) · 2024Article
- Comparative Proteomic Analysis of Ridge Gourd Seed (ACS omega · 2024Article
- Puzzle of Proteoform Variety-Where Is a Key?Proteomes · 2024Article
- How Can Proteomics Help to Elucidate the Pathophysiological Crosstalk in Muscular Dystrophy and Associated Multi-System Dysfunction?Proteomes · 2024Article
- Recent Advances in Metalloproteomics.Biomolecules · 2024Review
- Characterization of the plasma proteome from healthy adult dogs.Frontiers in veterinary science · 2024Article
- Proteomic methods for the study of porcine acute phase proteins - anything new to detect?Veterinary research communications · 2023Review
- Circulating Proteins as Diagnostic Markers in Gastric Cancer.International journal of molecular sciences · 2023Review
- Mass Spectrometry-Based Proteomic Technology and Its Application to Study Skeletal Muscle Cell Biology.Cells · 2023Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Two-dimensional gel electrophoresis was instrumental in the birth of proteomics in the late 1980s. However, it is now often considered as an outdated technique for proteomics-a thing of the past. Although this opinion may be true for some biological questions, e.g., when analysis depth is of critical importance, for many others, two-dimensional gel electrophoresis-based proteomics still has a lot to offer. This is because of its robustness, its ability to separate proteoforms, and its easy interface with many powerful biochemistry techniques (including western blotting). This paper reviews where and why two-dimensional gel electrophoresis-based proteomics can still be profitably used. It emerges that, rather than being a thing of the past, two-dimensional gel electrophoresis-based proteomics is still highly valuable for many studies. Thus, its use cannot be dismissed on simple fashion arguments and, as usual, in science, the tree is to be judged by the fruit.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.